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Literature summary for 2.4.2.18 extracted from
- Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus (2001), Eur. J. Biochem., 268, 2246-2252.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
| expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method | Saccharolobus solfataricus |
| hanging-drop method, crystals of ssTrpD diffract to better than 2.6 A resolution | Saccharolobus solfataricus |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is either digested by trypsin, V8-protease or thermolysin after incubation for 1 h at 25°C but not by thermolysin at 70°C | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000005 | - |
anthranilate | at 87°C, Km decreases at lower temperatures | Saccharolobus solfataricus |  |
| 0.002 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 25°C | Saccharolobus solfataricus | |
| 0.002 | - |
anthranilate | 25°C | Saccharolobus solfataricus | |
| 0.0026 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 40°C | Saccharolobus solfataricus | |
| 0.0026 | - |
anthranilate | 40°C | Saccharolobus solfataricus | |
| 0.0032 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 50°C | Saccharolobus solfataricus | |
| 0.0032 | - |
anthranilate | 50°C | Saccharolobus solfataricus | |
| 0.0035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 60°C | Saccharolobus solfataricus | |
| 0.0035 | - |
anthranilate | 60°C | Saccharolobus solfataricus | |
| 0.005 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 87°C | Saccharolobus solfataricus | |
| 0.005 | - |
anthranilate | 87°C | Saccharolobus solfataricus | |
| 0.01 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73300 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus | enzyme of tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus | P50384 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
| 95% pure | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme of tryptophan biosynthesis | Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ssTrpD | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
50% loss of activity after 69 min | Saccharolobus solfataricus |
| 70 | - |
t1/2: 69 min, addition of anthranilate has no stabilizing effect | Saccharolobus solfataricus |
| 85 | - |
50% loss of activity after 35 min | Saccharolobus solfataricus |
| 85 | - |
t1/2: 35 min, addition of anthranilate has no stabilizing effect | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 25°C | Saccharolobus solfataricus | |
| 0.014 | - |
anthranilate | 25°C | Saccharolobus solfataricus | |
| 0.014 | - |
anthranilate | at 25°C | Saccharolobus solfataricus | |
| 0.035 | - |
anthranilate | at 40°C | Saccharolobus solfataricus | |
| 0.035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 40°C | Saccharolobus solfataricus | |
| 0.035 | - |
anthranilate | 40°C | Saccharolobus solfataricus | |
| 0.067 | - |
anthranilate | at 50°C | Saccharolobus solfataricus | |
| 0.067 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 50°C | Saccharolobus solfataricus | |
| 0.067 | - |
anthranilate | 50°C | Saccharolobus solfataricus | |
| 0.105 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 60°C | Saccharolobus solfataricus | |
| 0.105 | - |
anthranilate | 60°C | Saccharolobus solfataricus | |
| 0.105 | - |
anthranilate | at 60°C | Saccharolobus solfataricus | |
| 0.4 | - |
anthranilate | at 87°C | Saccharolobus solfataricus | |
| 0.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 87°C | Saccharolobus solfataricus | |
| 0.4 | - |
anthranilate | 87°C | Saccharolobus solfataricus | |
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