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Literature summary for 2.4.2.18 extracted from

  • Marino, M.; Deuss, M.; Svergun, D.I.; Konarev, P.V.; Sterner, R.; Mayans, O.
    Structural and mutational analysis of substrate complexation by anthranilate phosphoribosyltransferase from Sulfolobus solfataricus (2006), J. Biol. Chem., 281, 21410-21421.
    View publication on PubMed
Search for more literature for 2.4.2.18

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of the wild-type enzyme complexed to its two natural substrates anthranilate and 5-phosphoribosyl-1-pyrophosphate/Mg2+ Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D223N kcat is 5.5fold higher than wild-type value at 2 mM Mg2+ Saccharolobus solfataricus
E224Q kcat is 5fold higher than wild-type value at 2 mM Mg2+ Saccharolobus solfataricus
H107A kcat is 1.2fold higher than wild-type value at 0.05 mM Mg2+ Saccharolobus solfataricus
H107A/P178A kcat is 2.1fold lower than wild-type value at 0.05 mM Mg2+ Saccharolobus solfataricus
K106Q kcat is 2.3fold lower than wild-type value at 2 mM Mg2+ Saccharolobus solfataricus
R164A kcat is 6.8fold lower than wild-type value at 0.05 mM Mg2+ Saccharolobus solfataricus
R164A/H154A kcat is 5.8fold lower than wild-type value at 0.05 mM Mg2+ Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
0.00007
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme D223N Saccharolobus solfataricus
0.00007
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
0.00016
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
0.00016
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme D223N Saccharolobus solfataricus
0.00017
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A Saccharolobus solfataricus
0.00026
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q Saccharolobus solfataricus
0.0003
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A Saccharolobus solfataricus
0.00053
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A Saccharolobus solfataricus
0.00075
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q Saccharolobus solfataricus
0.00091
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme E224Q Saccharolobus solfataricus
0.0026
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme E224Q Saccharolobus solfataricus
0.0043
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
0.0125
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A Saccharolobus solfataricus
0.149
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+,mutant enzyme R164A/H154A Saccharolobus solfataricus
0.297
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Saccharolobus solfataricus
-
 N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P50384
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
AnPRT
-
 Saccharolobus solfataricus
anthranilate PRT
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q Saccharolobus solfataricus
0.48
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q Saccharolobus solfataricus
0.62
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A Saccharolobus solfataricus
0.62
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A Saccharolobus solfataricus
0.73
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A/H154A Saccharolobus solfataricus
0.73
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+,mutant enzyme R164A/H154A Saccharolobus solfataricus
1.1
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
1.1
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
2
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A Saccharolobus solfataricus
2
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A Saccharolobus solfataricus
4.2
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
4.2
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme Saccharolobus solfataricus
5.1
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A Saccharolobus solfataricus
5.1
-
 anthranilate 60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A Saccharolobus solfataricus
5.6
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme E224Q Saccharolobus solfataricus
5.6
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme E224Q Saccharolobus solfataricus
6
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C, pH 7.5, 2 mM Mg2+, mutant enzyme D223N Saccharolobus solfataricus
6
-
 anthranilate 60°C, pH 7.5, 2 mM Mg2+,mutant enzyme D223N Saccharolobus solfataricus