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Literature summary for 2.4.2.19 extracted from
- Quinolinic acid phosphoribosyltransferase from castor bean endosperm. I. Purification and characterization (1974), J. Biol. Chem., 249, 6817-6823.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxynicotinate | 50% at 0.01 M | Ricinus communis |  |
| methyl-3-amidopyridine-2-carboxylate | - |
Ricinus communis | |
| methyl-3-cyanopyridine 2-carboxylate | - |
Ricinus communis | |
| NAD+ | 19% inhibition at 1 mM, 15% inhibition at 10 mM | Ricinus communis | |
| Picolinic acid | - |
Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
quinolinate | - |
Ricinus communis | |
| 0.045 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Ricinus communis |
| 68000 | - |
gel filtration | Ricinus communis |
| 72000 | - |
sucrose density gradient centrifugation | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | endosperm of etiolated seedlings | Ricinus communis | - |
| seedling | endosperm of etiolated seedlings | Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.75 | - |
- |
Ricinus communis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C, stable for several months in 0.05 M potassium phosphate buffer, pH 7.0, 50% sucrose w/v and 0.01 M dithiothreitol | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Ricinus communis | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Ricinus communis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quinolinate at 0.8 mM gives 50% protection against heat inactivation. 22% inhibition in presence of quinolinate | Ricinus communis |
| 55 | - |
10 min, 48% loss of activity | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.7 | - |
Ricinus communis |
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Release 2023.1 (January 2023)
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