Cloned (Comment) | Organism |
---|---|
into plasmid pTYB12 and expressed in Escherichia coli BL21 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
apo, quinolinate-bound, 5-phospho-alpha-D-ribose 1-diphosphate-bound, and phthalate-bound forms. Crystallized at room temperature by the hanging drop vapor diffusion method. Both apo and holo crystals belong to space group R32 (alpha = 90°, beta = 90°, and gamma = 120°). One molecule per asymmetric unit except in QAPRTase holophthalate crystals where two molecules are found. The unit cell dimensions are: a = b = 154.9 A and c = 68.9 A (apo), a = b = 154.8 A and c = 68.6 A (holoquinolinate), a = b = 154.9 A and c = 70.6 A (holo-5-phospho-alpha-D-ribose 1-diphosphate), a = b = 155.5 A and c = 121.1 A (holophthalate), a = b = 154.7 A and c = 69.3 A (holophthalate-5-phospho-alpha-D-ribose 1-diphosphate) | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phthalate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
191000 | - |
6 * 191000, dynamic light scattering analysis | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P43619 | - |
- |
Purification (Comment) | Organism |
---|---|
by anion exchange chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 191000, dynamic light scattering analysis | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
BNA6 | - |
Saccharomyces cerevisiae |
QAPRTase | - |
Saccharomyces cerevisiae |
quinolinate phosphoribosyl transferase | - |
Saccharomyces cerevisiae |