KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
uridine | - |
Geobacillus stearothermophilus | |
0.38 | - |
thymidine | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
sedimentation velocity analysis | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromodeoxyuridine + phosphate | 95% of the activity with uridine | Geobacillus stearothermophilus | 5-bromouracil + deoxyribose 1-phosphate | - |
? | |
5-bromouridine + phosphate | 95% of the activity with uridine | Geobacillus stearothermophilus | 5-bromouracil + alpha-D-ribose 1-phosphate | - |
? | |
cytidine + phosphate | 8% of the activity with uridine | Geobacillus stearothermophilus | cytosine + alpha-D-ribose 1-phosphate | - |
? | |
deoxyuridine + phosphate | 179% of the activity with uridine, by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed | Geobacillus stearothermophilus | uracil + 2-deoxy-D-ribose 1-phosphate | - |
r | |
additional information | no activity with deoxycytidine | Geobacillus stearothermophilus | ? | - |
? | |
thymidine + phosphate | 139% of the activity with uridine | Geobacillus stearothermophilus | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed | Geobacillus stearothermophilus | uracil + alpha-D-ribose 1-phosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, stable | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
uridine, thymidine | Geobacillus stearothermophilus |