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Literature summary for 2.4.2.2 extracted from

  • Tran, T.H.; Christoffersen, S.; Allan, P.W.; Parker, W.B.; Piskur, J.; Serra, I.; Terreni, M.; Ealick, S.E.
    The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases (2011), Biochemistry, 50, 6549-6558.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with the products, ribose 1-phosphate and uracil, at 1.8 A resolution. The biological unit is a hexamer with an alpha/beta monomeric fold. Residue His169 structurally aligns with Arg168 of the Escherichia coli uridine phosphorylase structure. A second active site residue, Lys162, is not present in previously determined uridine phosphorylase structures and interacts with O2 of uracil Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
H169A about 10% of wild-type activity Streptococcus pyogenes
H169D about 1% of wild-type activity Streptococcus pyogenes
H169N about 4% of wild-type activity Streptococcus pyogenes
K162A complete loss of activity Streptococcus pyogenes
V220D almost complete loss of activity Streptococcus pyogenes
V220E less than 0.5% of wild-type activity Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.158
-
 uridine wild-type, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
0.171
-
 uridine mutant H169A, pH 7.5, temperature not specified in the publication Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes Q5XA29
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
 substrate thymidine, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
8.1
-
 substrate uridine, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
11.2
-
 substrate deoxyuridine, pH 7.5, temperature not specified in the publication Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxyuridine + phosphate
-
 Streptococcus pyogenes uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
additional information no substrate: cytidine, deoxycytidine, adenosine, inosine, or guanosine Streptococcus pyogenes ?
-
 ?
thymidine + phosphate
-
 Streptococcus pyogenes thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
uridine + phosphate preferred substrate Streptococcus pyogenes uracil + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 uridine mutant H169A, pH 7.5, temperature not specified in the publication Streptococcus pyogenes
15
-
 uridine wild-type, pH 7.5, temperature not specified in the publication Streptococcus pyogenes