Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Parageobacillus thermoglucosidasius |
expression in Escherichia coli | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
uridine | pH 7.0, 60°C | Thermus thermophilus | |
0.43 | - |
thymidine | pH 7.0, 60°C | Thermus thermophilus | |
1.3 | - |
thymidine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius | |
2.3 | - |
uridine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | - |
- |
- |
Thermus thermophilus | Q72HS4 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72HS4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate | 1.6% substrate conversion after 30 min | ? | |
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate | 1.6% substrate conversion after 30 min | ? | |
2'-deoxy-2'-fluorouridine + phosphate | - |
Parageobacillus thermoglucosidasius | ? | poor activity, 0.4% substrate conversion after 30 min | ? | |
2'-deoxy-2'-fluorouridine + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate | 15.6% substrate conversion after 30 min | ? | |
2'-deoxy-2'-fluorouridine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate | 15.6% substrate conversion after 30 min | ? | |
thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | - |
Thermus thermophilus | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | prefered substrate | Parageobacillus thermoglucosidasius | uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | prefered substrate | Thermus thermophilus | uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | prefered substrate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | uracil + alpha-D-ribose 1-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life 1.6 h | Parageobacillus thermoglucosidasius |
70 | - |
half-life 1.6 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83 | - |
thymidine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius | |
277 | - |
uridine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius | |
277 | - |
uridine | pH 7.0, 60°C | Thermus thermophilus | |
679 | - |
thymidine | pH 7.0, 60°C | Thermus thermophilus |