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Literature summary for 2.4.2.2 extracted from

  • Szeker, K.; Zhou, X.; Schwab, T.; Casanueva, A.; Cowan, D.; Mikhailopulo, I.; Neubauer, P.
    Comparative investigations on thermostable pyrimidine nucleoside phosphorylases from Geobacillus thermoglucosidasius and Thermus thermophilus (2012), J. Mol. Catal. B, 84, 27-34.
No PubMed abstract available
Search for more literature for 2.4.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Parageobacillus thermoglucosidasius
expression in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 uridine pH 7.0, 60°C Thermus thermophilus
0.43
-
 thymidine pH 7.0, 60°C Thermus thermophilus
1.3
-
 thymidine pH 7.0, 60°C Parageobacillus thermoglucosidasius
2.3
-
 uridine pH 7.0, 60°C Parageobacillus thermoglucosidasius

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius
-
-
-
Thermus thermophilus Q72HS4
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72HS4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
-
 Thermus thermophilus uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate 1.6% substrate conversion after 30 min ?
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate 1.6% substrate conversion after 30 min ?
2'-deoxy-2'-fluorouridine + phosphate
-
 Parageobacillus thermoglucosidasius ? poor activity, 0.4% substrate conversion after 30 min ?
2'-deoxy-2'-fluorouridine + phosphate
-
 Thermus thermophilus uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate 15.6% substrate conversion after 30 min ?
2'-deoxy-2'-fluorouridine + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate 15.6% substrate conversion after 30 min ?
thymidine + phosphate
-
 Parageobacillus thermoglucosidasius thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
thymidine + phosphate
-
 Thermus thermophilus thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
thymidine + phosphate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
uridine + phosphate prefered substrate Parageobacillus thermoglucosidasius uracil + alpha-D-ribose 1-phosphate
-
 ?
uridine + phosphate prefered substrate Thermus thermophilus uracil + alpha-D-ribose 1-phosphate
-
 ?
uridine + phosphate prefered substrate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 uracil + alpha-D-ribose 1-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life 1.6 h Parageobacillus thermoglucosidasius
70
-
 half-life 1.6 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83
-
 thymidine pH 7.0, 60°C Parageobacillus thermoglucosidasius
277
-
 uridine pH 7.0, 60°C Parageobacillus thermoglucosidasius
277
-
 uridine pH 7.0, 60°C Thermus thermophilus
679
-
 thymidine pH 7.0, 60°C Thermus thermophilus