Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 84% activation at 25 mM | Homo sapiens | |
histone protein | activity gains 1.5fold when histone protein is added at a concentration of 0.1 mg/ml | Homo sapiens | |
protamine | adding protamine to a final concentration of 0.1 mg/ml stimulates XT-II activity at least 2fold | Homo sapiens | |
Sodium citrate | 72% activation at 25 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | XT-II activity is completely abolished at a concentration 1% (v/v) beta-mercaptoethanol | Homo sapiens | |
CDP | 23% residual activity at 10 mg/ml | Homo sapiens | |
CMP | 45% residual activity at 10 mg/ml | Homo sapiens | |
Co2+ | Co2+ reduces activity by more than 60% at 5 mM | Homo sapiens | |
CTP | 8% residual activity at 10 mg/ml | Homo sapiens | |
Cu2+ | complete inhibition at 5 mM | Homo sapiens | |
dithiothreitol | XT-II activity is completely abolished at a concentration of 1 mM dithiothreitol | Homo sapiens | |
additional information | lysozyme does not change the activity of XT-II at any concentration | Homo sapiens | |
Ni2+ | complete inhibition at 5 mM | Homo sapiens | |
protamine | adding protamine to a final concentration of 10 mg/ml decreases XT-II activity 4fold | Homo sapiens | |
UDP | 5% residual activity at 10 mg/ml | Homo sapiens | |
UMP | 25% residual activity at 10 mg/ml | Homo sapiens | |
UTP | 4% residual activity at 10 mg/ml | Homo sapiens | |
Zn2+ | complete inhibition at 5 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
Bio-QEEEGSGGGQKK-F | - |
Homo sapiens | |
0.0108 | - |
bikunin | - |
Homo sapiens | |
0.0191 | - |
fibroblast growth factor 2 fragment 1-24 | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | necessity for Mg2+ or Mn2+ ions for enzymatic activity | Homo sapiens | |
Mn2+ | necessity for Mg2+ or Mn2+ ions for enzymatic activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H1B5 | - |
- |
Storage Stability | Organism |
---|---|
-80°C, -20°C or 4°C, 3-4 weeks, no loss of activity | Homo sapiens |
25°C, 2 weeks, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | TENEGSGLTNIK, SENEGSGMAEQK, orcokinin (NFDEIDRSGFGFN), melittin (GIGAVLKVLTTGLPALISWIKRKRQQ), fibrinopeptide A (ADSGEGDFLAEGGGVR), IgE C4 domain (KTKGSGFFVF), C-type natriuretic peptide (GLSKGCFGLKLDRIGSMSGLGC), and calcitonin (VLGKLSQELHKLQTYPRTNTGSGTP) are no substrates for XT-II | Homo sapiens | ? | - |
? | |
UDP-D-xylose + bikunin | - |
Homo sapiens | UDP + D-xylosyl-bikunin | - |
? | |
UDP-D-xylose + biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 | the bikunin-homologous peptide Bio-QEEEGSGGGQKK-F is best acceptor substrate for XT-II, 100% activity with UDP-D-xylose | Homo sapiens | UDP + biotin-NH-QEEEG-(D-xylosyl)SGGGQKK(5-fluorescein)-CONH2 | - |
? | |
UDP-D-xylose + fibroblast growth factor 2 fragment 1-24 | - |
Homo sapiens | ? | - |
? | |
UDP-D-xylose + silk fibroin | - |
Homo sapiens | UDP + silk fibroin with xylosylserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XT-II | - |
Homo sapiens |
xylosyltransferase II | isoform of xylosyltransferase I | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
reaching a temperature of 37°C, XT-II has 66% of the activity compared with 30°C | Homo sapiens |