Cloned (Comment) | Organism |
---|---|
into pGEX-6P-1, ligated into the multiple cloning site of the BamHI-digested and shrimp alkaline phosphatase-dephosphorylated pMAL-c4E expression vector, in frame with the N-terminal located maltose binding protein, expressed in Escherichia coli strain BL21(DE3) under the control of the IPTG inducible tac promoter. MBP/XylT-II fusion protein expressed in ER2507 that contains prolonged XylT-II fragments (MBP/XylT-IIF1-3 and MBP/XylT-IIF4-6) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
full-length XylT-II purified to homogeneity as inclusion bodies, by maltose binding protein affinity chromatography and heparin affinity chromatography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
xylosyltransferase II | - |
Homo sapiens |
XylT-II | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | XylT-II fragments bind with low affinity to heparin. Prolonging of XylT-II fragments does not account for a cooperative effect of multiple heparin-binding motifs and in turn for a stronger heparin-binding. Two high polarity surface regions in the stem region with the sequence [-K-G-R-Q-R-K-P-R-P-] and [-G-R-R-H-G-R-W-], both representing Cardin-Weintraub motifs | Homo sapiens |