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Literature summary for 2.4.2.28 extracted from

  • Cacciapuoti, G.; Forte, S.; Moretti, M.A.; Brio, A.; Zappia, V.; Porcelli, M.
    A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus (2005), FEBS J., 272, 1886-1899.
    View publication on PubMed
Search for more literature for 2.4.2.28

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
C259S/C261S mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme Saccharolobus solfataricus
C262S mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0254
-
 adenosine pH 7.4, 70°C Saccharolobus solfataricus
0.084
-
 Inosine pH 7.4, 70°C Saccharolobus solfataricus
0.1136
-
 guanosine pH 7.4, 70°C Saccharolobus solfataricus
0.154
-
 5'-methylthioadenosine pH 7.4, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 6 * 30000, SDS-PAGE Saccharolobus solfataricus
180000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + phosphate
-
 Saccharolobus solfataricus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
adenosine + phosphate
-
 Saccharolobus solfataricus adenine + D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Saccharolobus solfataricus guanine + D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Saccharolobus solfataricus hypoxanthine + D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 30000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
5'-deoxy-5'-methylthioadenosine phosphorylase II
-
 Saccharolobus solfataricus
SsMTAPII
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
120
-
 wild-type enzyme Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
95 140 95°C: about 50% of maximal activity, 140°C: about 45% of maximal activity, wild-type enzyme Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 1 h, wild-type enzyme is completely stable, mutant enzyme C262S loses 32% of its activity, mutant enzyme c259S/C261A loses 63% of its activity Saccharolobus solfataricus
102
-
 apparent Tm, mutant enzyme C259S/C261S Saccharolobus solfataricus
106
-
 apparent Tm, mutant enzyme C262S Saccharolobus solfataricus
112
-
 apparent Tm, wild-type enzyme Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.4
-
 5'-methylthioadenosine pH 7.4, 70°C Saccharolobus solfataricus
29.6
-
 guanosine pH 7.4, 70°C Saccharolobus solfataricus
43.6
-
 adenosine pH 7.4, 70°C Saccharolobus solfataricus
132
-
 Inosine pH 7.4, 70°C Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus calculation from nucleotide sequence
-
 6.54