Literature summary for 2.4.2.28 extracted from

Buckoreelall, K.; Sun, Y.; Hobrath, J.V.; Wilson, L.; Parker, W.B.
Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis (2012), Tuberculosis, 92, 139-147.

Search for more literature for 2.4.2.28

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-methylthio-DADMe-immucillin A	-	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009	-	5'-methylthioadenosine	pH and temperature not specified in the publication, co-substrate: 50 mM phosphate	Mycobacterium tuberculosis
0.26	-	phosphate	pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	SDS-PAGE	Mycobacterium tuberculosis
60000	-	gel filtration	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O06401	-	-

Storage Stability

Storage Stability	Organism
-20°C, stable	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-methylthioadenosine + phosphate	-	Mycobacterium tuberculosis	adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate	-	?
adenosine + phosphate	2.1% activity compared to activity with 5'-methylthioadenosine	Mycobacterium tuberculosis	adenine + D-ribose 1-phosphate	-	?
S-adenosyl-L-homocysteine + phosphate	0.81% activity compared to activity with 5'-methylthioadenosine	Mycobacterium tuberculosis	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 30000, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
MTAP	-	Mycobacterium tuberculosis
Rv0535	-	Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	4h, 40% loss of activity	Mycobacterium tuberculosis
37	-	4h, 90% loss of activity	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.26	-	phosphate	pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine	Mycobacterium tuberculosis
0.4	-	5'-methylthioadenosine	pH and temperature not specified in the publication, co-substrate: 50 mM phosphate	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	7-7.5 optimal pH	Mycobacterium tuberculosis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	9	pH 6.5: about 55% of maximal activity, pH 9.0: about 55% of maximal activity	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000025	-	pH and temperature not specified in the publication, in the presence of 10 microM 5'-methylthioadenosine	Mycobacterium tuberculosis	5'-methylthio-DADMe-immucillin A
0.000075	-	pH and temperature not specified in the publication, in the presence of 100 microM 5'-methylthioadenosine	Mycobacterium tuberculosis	5'-methylthio-DADMe-immucillin A

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Release 2023.1 (January 2023)