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Literature summary for 2.4.2.28 extracted from

  • Vodnala, M.; Ranjbarian, F.; Pavlova, A.; de Koning, H.P.; Hofer, A.
    Trypanosoma brucei methylthioadenosine phosphorylase protects the parasite from the antitrypanosomal effect of deoxyadenosine: implications for the pharmacology of adenosine antimetabolites (2016), J. Biol. Chem., 291, 11717-11726.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not dependent on dithiothreitol Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
adenine
-
Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00009
-
S-methyl-5'-thioadenosine at pH 7.4 and 37°C Trypanosoma brucei
0.005
-
2'-fluoro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
0.0083
-
deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
0.013
-
adenosine at pH 7.4 and 37°C Trypanosoma brucei
0.032
-
2'-chloro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not dependent on Na+, K+, or Mg2+ Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-methyl-5'-thioadenosine + phosphate Trypanosoma brucei
-
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?
S-methyl-5'-thioadenosine + phosphate Trypanosoma brucei TC221
-
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-
Trypanosoma brucei TC221
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex G-25 gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-chloro-2'-deoxyadenosine + phosphate
-
Trypanosoma brucei adenine + 2-chloro-2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-chloro-2'-deoxyadenosine + phosphate
-
Trypanosoma brucei TC221 adenine + 2-chloro-2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-fluoro-2'-deoxyadenosine + phosphate
-
Trypanosoma brucei adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate
-
?
2'-fluoro-2'-deoxyadenosine + phosphate
-
Trypanosoma brucei TC221 adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate
-
?
adenosine + phosphate
-
Trypanosoma brucei adenine + D-ribose 1-phosphate
-
?
adenosine + phosphate
-
Trypanosoma brucei TC221 adenine + D-ribose 1-phosphate
-
?
deoxyadenosine + phosphate
-
Trypanosoma brucei adenine + deoxy-D-ribose 1-phosphate
-
?
additional information very low activity with cordycepin, Ara-A, tubercidin, guanosine, deoxyguanosine, inosine, deoxyinosine, uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine Trypanosoma brucei ?
-
?
additional information very low activity with cordycepin, Ara-A, tubercidin, guanosine, deoxyguanosine, inosine, deoxyinosine, uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine Trypanosoma brucei TC221 ?
-
?
S-methyl-5'-thioadenosine + phosphate
-
Trypanosoma brucei adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?
S-methyl-5'-thioadenosine + phosphate
-
Trypanosoma brucei TC221 adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
methylthioadenosine phosphorylase
-
Trypanosoma brucei
MTAP
-
Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.13
-
S-methyl-5'-thioadenosine at pH 7.4 and 37°C Trypanosoma brucei
5.4
-
2'-fluoro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
6.4
-
2'-chloro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
8.4
-
adenosine at pH 7.4 and 37°C Trypanosoma brucei
9.6
-
deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8 the enzyme activity is stable between pH 5.5 and 8.0 Trypanosoma brucei

General Information

General Information Comment Organism
malfunction there is increased deoxyadenosine sensitivity in Trypanosoma brucei methylthioadenosine phosphorylase knockdown cells Trypanosoma brucei
physiological function the enzyme protects Trypanosoma brucei from the antitrypanosomal effect of deoxyadenosine Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
200
-
2'-chloro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
650
-
adenosine at pH 7.4 and 37°C Trypanosoma brucei
1080
-
2'-fluoro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
1160
-
deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
24000
-
S-methyl-5'-thioadenosine at pH 7.4 and 37°C Trypanosoma brucei