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Literature summary for 2.4.2.3 extracted from
- Purification and substrate specificity of pyrimidine nucleoside phosphorylase from Haemophilus influenzae (1971), J. Biol. Chem., 246, 6606-6610.
General Stability
| General Stability | Organism |
|---|---|
| use of dithiothreitol and glycerol is necessary for stabilization during purification | Haemophilus influenzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
5-Bromouridine | - |
Haemophilus influenzae |  |
| 0.07 | - |
5-methyluridine | - |
Haemophilus influenzae | |
| 0.1 | - |
5-bromodeoxyuridine | - |
Haemophilus influenzae | |
| 0.11 | - |
thymidine | - |
Haemophilus influenzae | |
| 0.13 | - |
2'-deoxyuridine | - |
Haemophilus influenzae | |
| 0.24 | - |
uridine | - |
Haemophilus influenzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 400fold | Haemophilus influenzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 510 | - |
- |
Haemophilus influenzae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 6 months | Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyuridine + phosphate | 12% of the activity with uridine | Haemophilus influenzae | uracil + deoxyribose 1-phosphate | - |
? | |
| 5-bromo-2'-deoxyuridine + phosphate | 75% of the activity with uridine | Haemophilus influenzae | 5-bromouracil + 2-deoxribose 1-phosphate | - |
? | |
| 5-bromouracil + deoxyribose 1-phosphate | - |
Haemophilus influenzae | 5-bromodeoxyuridine + phosphate | - |
? | |
| 5-bromouridine + phosphate | 40% of the activity with uridine | Haemophilus influenzae | 5-bromouracil + alpha-D-ribose 1-phosphate | - |
? | |
| 5-methyluridine + phosphate | 27% of the activity with uridine | Haemophilus influenzae | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
| additional information | no substrate: cytidine | Haemophilus influenzae | ? | - |
? | |
| thymidine + phosphate | 21% of the activity with uridine | Haemophilus influenzae | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| uracil arabinoside + phosphate | 10% of the activity with uridine | Haemophilus influenzae | uracil + arabinose-1-phosphate | - |
? | |
| uridine + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-ribose 1-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
deoxynucleoside phosphorolysis | Haemophilus influenzae |
| 7.4 | - |
phosphorolysis of ribonucleosides and uracil arabinoside | Haemophilus influenzae |
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Release 2023.1 (January 2023)
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