Literature summary for 2.4.2.3 extracted from

Safonova, T.N.; Mordkovich, N.N.; Veiko, V.P.; Okorokova, N.A.; Manuvera, V.A.; Dorovatovskii, P.V.; Popov, V.O.; Polyakov, K.M.
Concerted action of two subunits of the functional dimer of Shewanella oneidensis MR-1 uridine phosphorylase derived from a comparison of the C212S mutant and the wild-type enzyme (2016), Acta Crystallogr. Sect. D, 72, 203-210.

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Cloned(Commentary)

Cloned (Comment)	Organism
mutant C212S is expressed in Escherichia coli JM110 cells	Shewanella oneidensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme C212S, hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Tris pH 8.5, and 25% (w/v) PEG 3350	Shewanella oneidensis

Protein Variants

Protein Variants	Comment	Organism
C212S	the mutation accounts for the lower affinity of the mutant for inorganic phosphate, while the affinity for uridine remains unchanged	Shewanella oneidensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	uridine	wild type enzyme, at pH 7.4 and 37°C	Shewanella oneidensis
0.24	-	uridine	mutant enzyme C212S, at pH 7.4 and 37°C	Shewanella oneidensis
5.1	-	phosphate	wild type enzyme, at pH 7.4 and 37°C	Shewanella oneidensis
16.1	-	phosphate	mutant enzyme C212S, at pH 7.4 and 37°C	Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uridine + phosphate	Shewanella oneidensis	-	uracil + alpha-D-ribose 1-phosphate	-	?
uridine + phosphate	Shewanella oneidensis MR-1 / ATCC 700550	-	uracil + alpha-D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Shewanella oneidensis	C7EWM3	-	-
Shewanella oneidensis MR-1 / ATCC 700550	C7EWM3	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Shewanella oneidensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uridine + phosphate	-	Shewanella oneidensis	uracil + alpha-D-ribose 1-phosphate	-	?
uridine + phosphate	-	Shewanella oneidensis MR-1 / ATCC 700550	uracil + alpha-D-ribose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	-	Shewanella oneidensis

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Release 2023.1 (January 2023)