Literature summary for 2.4.2.30 extracted from

Liu, S.; Kulich, S.M.; Barbieri, J.T.
Identification of glutamic acid 381 as a candidate active site residue of Pseudomonas aeruginosa exoenzyme S (1996), Biochemistry, 35, 2754-2758.

Search for more literature for 2.4.2.30

Cloned(Commentary)

Cloned (Comment)	Organism
catalytic domain DELTAN22 and DELTAN222 mutants, expression in Escherichia coli	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
DELTAN222	the carboxy-terminal 222 amino acids, catalytic domain	Pseudomonas aeruginosa
DELTAN222/E381A	possesses 0.02% of the ADP-ribosyltransferase activity of DELTAN222. KM-value for (ADP-D-ribosyl)n-soybean-trypsin-inhibitor is 8.7fold higher than the KM-value for DELTAN222	Pseudomonas aeruginosa
DELTAN222/E381D	possesses 0.6% of the ADP-ribosyltransferase activity of DELTAN222. KM-value for (ADP-D-ribosyl)n-soybean-trypsin-inhibitor is 7.9fold higher than the KM-value for DELTAN222	Pseudomonas aeruginosa
DELTAN222/E381S	possesses 0.01% of the ADP-ribosyltransferase activity of DELTAN222. KM-value for (ADP-D-ribosyl)n-soybean-trypsin-inhibitor is 4.7fold higher than the KM-value for DELTAN222	Pseudomonas aeruginosa
DELTAN222/E387A	possesses 31% of the ADP-ribosyltransferase activity of DELTAN222	Pseudomonas aeruginosa
DELTAN222/E399A	possesses 28% of the ADP-ribosyltransferase activity of DELTAN222	Pseudomonas aeruginosa
DELTAN222/E414A	possesses 20% of the ADP-ribosyltransferase activity of DELTAN222	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Pseudomonas aeruginosa
0.049	-	(ADP-D-ribosyl)n-soybean-trypsin-inhibitor	DELTAN222	Pseudomonas aeruginosa
0.23	-	(ADP-D-ribosyl)n-soybean-trypsin-inhibitor	DELTAN222/E381S	Pseudomonas aeruginosa
0.387	-	(ADP-D-ribosyl)n-soybean-trypsin-inhibitor	DELTAN222/E381D	Pseudomonas aeruginosa
0.429	-	(ADP-D-ribosyl)n-soybean-trypsin-inhibitor	DELTAN222/E381A	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic domain DELTAN22 and DELTAN222 mutants, expression in Escherichia coli	Pseudomonas aeruginosa

Storage Stability

Storage Stability	Organism
-20°C, 40% glycerol, stable for several months	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (ADP-D-ribosyl)n-soybean-trypsin-inhibitor	-	Pseudomonas aeruginosa	nicotinamide + (ADP-D-ribosyl)n+1-soybean-trypsin-inhibitor	-	?

Synonyms

Synonyms	Comment	Organism
exoenzyme S	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)