Literature summary for 2.4.2.30 extracted from

Faraone Mennella, M.R.; Castellano, S.; De Luca, P.; Discenza, A.; Gambacorta, A.; Nicolaus, B.; Farina, B.
Comparison of the ADP-ribosylating thermozyme from Sulfolobus solfataricus and the mesophilic poly(ADP-ribose) polymerases (2000), FEMS Microbiol. Lett., 192, 9-14.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Mg2+	5 mM, 6.3fold activation	Saccharolobus solfataricus
Phenanthroline	0.1 mM, 1.1fold activation	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
3-aminobenzamide	10 mM, 47% inhibition; 10 mM, 47% inhibition	Saccharolobus solfataricus
additional information	phenanthroline has no or a slightly activating effect	Saccharolobus solfataricus
nicotinamide	10 mM, 14% inhibition; 10 mM, 14% inhibition	Saccharolobus solfataricus
Zn2+	0.5 mM, 47% inhibition; 0.5 mM, 50% inhibition	Saccharolobus solfataricus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	0.1 mM, 4.1fold activation	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46500	-	gel filtration	Saccharolobus solfataricus
50000	-	1 * 50000, SDS-PAGE	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	i.e. DSM5833	-
Saccharolobus solfataricus	B3EWG9	-	-
Saccharolobus solfataricus MT-4 / DSM 5833	-	i.e. DSM5833	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (ADP-D-ribosyl)n-acceptor	-	Saccharolobus solfataricus	nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 50000, SDS-PAGE	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
ADP-ribosylating thermozyme	-	Saccharolobus solfataricus
PARPss	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Saccharolobus solfataricus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Saccharolobus solfataricus	-	7.2	7
Saccharolobus solfataricus	isoelectric focusing	7.2	7

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Release 2023.1 (January 2023)