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Literature summary for 2.4.2.31 extracted from
- Comparison of acceptor protein specificities on the formation of ADP-ribose.acceptor adducts by arginine-specific ADP-ribosyltransferase from rabbit skeletal muscle sarcoplasmic reticulum with those of the enzyme from chicken peripheral polymorphonuclear cells (1993), Biochim. Biophys. Acta, 1161, 265-271.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DNA | enhances activity | Gallus gallus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K2HPO4 | IC50: 50 mM | Gallus gallus | |
| K2HPO4 | IC50: 40 mM | Oryctolagus cuniculus | |
| NaCl | IC50: 50 mM | Gallus gallus | |
| NaCl | IC50: 100 mM | Oryctolagus cuniculus | |
| novobiocin | IC50: 0.2 mM | Gallus gallus | |
| novobiocin | IC50: 0.4 mM | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
NAD+ | - |
Gallus gallus | |
| 0.2 | - |
NAD+ | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| sarcoplasmic reticulum | - |
Oryctolagus cuniculus | 16529 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
x * 27500, SDS-PAGE | Oryctolagus cuniculus |
| 40000 | - |
x * 40000, SDS-PAGE | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| granulocyte | peripheral polymorphonuclear | Gallus gallus | - |
| skeletal muscle | - |
Gallus gallus | - |
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.32 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| actin + NAD+ | no activity with the enzyme from skeletal muscle sarcoplasmic reticulum | Oryctolagus cuniculus | nicotinamide + ? | - |
? | |
| actin + NAD+ | alpha-actin and beta/gamma-actin | Gallus gallus | nicotinamide + ? | - |
? | |
| casein + NAD+ | - |
Gallus gallus | nicotinamide + ? | - |
? | |
| P33 + NAD+ | preferential endogenous acceptor | Gallus gallus | nicotinamide + ? | - |
? | |
| P33 + NAD+ | no activity with the enzyme from skeletal muscle sarcoplasmic reticulum | Oryctolagus cuniculus | nicotinamide + ? | - |
? | |
| polyarginine + NAD+ | - |
Gallus gallus | nicotinamide + ? | - |
? | |
| polyarginine + NAD+ | - |
Oryctolagus cuniculus | nicotinamide + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Gallus gallus |
| ? | x * 40000, SDS-PAGE | Oryctolagus cuniculus |
| ? | x * 27500, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Gallus gallus |
| 9 | - |
- |
Oryctolagus cuniculus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
IC50: 0.2 mM | Gallus gallus | novobiocin | |
| 0.4 | - |
IC50: 0.4 mM | Oryctolagus cuniculus | novobiocin | |
| 40 | - |
IC50: 40 mM | Oryctolagus cuniculus | K2HPO4 | |
| 50 | - |
IC50: 50 mM | Gallus gallus | NaCl | |
| 50 | - |
IC50: 50 mM | Gallus gallus | K2HPO4 | |
| 100 | - |
IC50: 100 mM | Oryctolagus cuniculus | NaCl | |
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Release 2023.1 (January 2023)
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