Literature summary for 2.4.2.36 extracted from

Gong, Z.; Long, X.; Pan, L.; Le, Y.; Liu, Q.; Wang, S.; Guo, J.; Xiao, B.; Zhou, M.; Mei, D.
Cloning, expression, purification and characterization of the cholera toxin B subunit and triple glutamic acid decarboxylase epitopes fusion protein in Escherichia coli (2009), Protein Expr. Purif., 191, 191-197.

Search for more literature for 2.4.2.36

Application

Application	Comment	Organism
medicine	therapy for autoimmune diabetes with glutamic acid decarboxylase 65 as autoantigen combined with cholera toxin B subunit as adjuvans lowering the number of antigens needed for autoimmune suppression, oral application for 5 weeks reduces insulitis of pancreas in mouse model	Vibrio cholerae serotype O1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) with expression vector pET28a	Vibrio cholerae serotype O1

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant gene of cholera toxin B subunit with triple glutamic acid decarboxylase 65 peptides 531-545 (3p531) with 6-bp oligonucleotide sequences as flexible hinges	Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	SDS-PAGE of purified recombinant fusion enzyme cholera toxin B subunit with glutamic acid carboxylase 65 peptides 531-545 with histidine 6 tag	Vibrio cholerae serotype O1

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
adsorption on Ni-NTA resin column, washing and elution with buffer containing 500 mM imidazole, dialyzing in 2 steps	Vibrio cholerae serotype O1

Synonyms

Synonyms	Comment	Organism
CTB	cholera toxin B subunit	Vibrio cholerae serotype O1

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Release 2023.1 (January 2023)