Cloned (Comment) | Organism |
---|---|
expression of N-terminally His5-tagged enzyme in Pichia pastoris, recombinant enzyme is secreted to the medium | Arabidopsis thaliana |
General Stability | Organism |
---|---|
the purified recombinant enzyme is not stable to a single freeze-thaw cycle | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His5-tagged enzyme from Pichia pastoris cell medium by nickel chelating affinity chromatography and ultrafiltration to homogeneity | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
purified recombinant enzyme | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme, 3 weeks stable without loss of activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc | i.e. dansylated GnGn-peptide | Arabidopsis thaliana | UDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
beta1,2-xylosyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Arabidopsis thaliana |