General Stability | Organism |
---|---|
2-mercaptoethanol and sucrose stabilize during preincubation procedure with ammonium sulfate | Salmonella enterica subsp. enterica serovar Typhimurium |
quite labile during purification | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-D-ribose | product inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
5-Hydroxymethyluracil | 35% inactivation at 2 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Bromouracil | 100% inactivation at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
deoxyadenosine | 20% inactivation at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
ribose 1-phosphate | 20% inactivation at 1.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
thymine | product inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
Uracil | 84% inactivation at 2 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
uridine | competitive 27% inactivation at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.1 | - |
thymidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.3 | - |
phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
8 | - |
deoxyuridine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, SDS-disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
100000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
Purification (Comment) | Organism |
---|---|
257fold | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate | ordered bi bi mechanism with the nucleoside the first substrate to add, and the pyrimidine base the last product to leave | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
465 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, 10 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol, 20% sucrose, stable for 3 months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bromodeoxyuridine + phosphate | at 40% the rate of thymidine | Salmonella enterica subsp. enterica serovar Typhimurium | bromouracil + 2-deoxy-D-ribose | - |
? | |
deoxyuridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
iododeoxyuridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | iodouracil + 2-deoxy-D-ribose | - |
? | |
additional information | no substrate: deoxycytidine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | no substrates: deoxyadenosine, deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | specific for deoxyribonucleosides | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
thymidine + arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
thymidine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, SDS-disc gel electrophoresis | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | rapid decrease of activity above and below this range | Salmonella enterica subsp. enterica serovar Typhimurium |