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Literature summary for 2.4.2.48 extracted from

  • Watanabe, M.; Matsuo, M.; Tanaka, S.; Akimoto, H.; Asahi, S.; Nishimura, S.; Katze, J.R.; Hashizume, T.; Crain, P.F.; McCloskey, J.A.; Okada, N.
    Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to archaeal tRNA, proceeds via a pathway involving base replacement on the tRNA polynucleotide chain (1997), J. Biol. Chem., 272, 20146-20151.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ may be responsible for conformational rigidity of the tRNA, but not for the enzymatic activity itself. Mg2+ ion is required for activity with the T7 transcript as a substrate. Activity with unfractionated Escherichia coli tRNA does not require Mg2+ Haloferax volcanii
additional information high salt concentration approximately 2.4 M is necessary for maximum activity Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
x * 78000, SDS-PAGE Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine15 in tRNA + 7-cyano-7-carbaguanine Haloferax volcanii key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?
guanine15 in tRNA + 7-cyano-7-carbaguanine Haloferax volcanii ATCC 29605 key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii Q9C4M3
-
-
Haloferax volcanii ATCC 29605 Q9C4M3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine15 in tRNA + 7-cyano-7-carbaguanine key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs Haloferax volcanii 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?
guanine15 in tRNA + 7-cyano-7-carbaguanine archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction Haloferax volcanii 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?
guanine15 in tRNA + 7-cyano-7-carbaguanine key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs Haloferax volcanii ATCC 29605 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?
guanine15 in tRNA + 7-cyano-7-carbaguanine archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction Haloferax volcanii ATCC 29605 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Haloferax volcanii

Synonyms

Synonyms Comment Organism
tRNA-guanine transglycosylase
-
Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Haloferax volcanii