Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ may be responsible for conformational rigidity of the tRNA, but not for the enzymatic activity itself. Mg2+ ion is required for activity with the T7 transcript as a substrate. Activity with unfractionated Escherichia coli tRNA does not require Mg2+ | Haloferax volcanii | |
additional information | high salt concentration approximately 2.4 M is necessary for maximum activity | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Haloferax volcanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine15 in tRNA + 7-cyano-7-carbaguanine | Haloferax volcanii | key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? | |
guanine15 in tRNA + 7-cyano-7-carbaguanine | Haloferax volcanii ATCC 29605 | key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | Q9C4M3 | - |
- |
Haloferax volcanii ATCC 29605 | Q9C4M3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine15 in tRNA + 7-cyano-7-carbaguanine | key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs | Haloferax volcanii | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? | |
guanine15 in tRNA + 7-cyano-7-carbaguanine | archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction | Haloferax volcanii | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? | |
guanine15 in tRNA + 7-cyano-7-carbaguanine | key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs | Haloferax volcanii ATCC 29605 | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? | |
guanine15 in tRNA + 7-cyano-7-carbaguanine | archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction | Haloferax volcanii ATCC 29605 | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
tRNA-guanine transglycosylase | - |
Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Haloferax volcanii |