Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.52 extracted from

  • Schneider, K.; Dimroth, P.; Bott, M.
    Biosynthesis of the prosthetic group of citrate lyase (2000), Biochemistry, 39, 9438-9450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
? * 32600, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3'-dephospho-CoA Escherichia coli
-
2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
strain JM83, strain CM2080, gene citG
-
Escherichia coli P77231
-
-
Klebsiella pneumoniae P45414
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Storage Stability

Storage Stability Organism
-4°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3'-dephospho-CoA
-
Escherichia coli 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine
-
?
ATP + 3'-dephospho-CoA ATP cannot be replaced by CTP, GTP or UTP Escherichia coli 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine
-
?
additional information holo-acyl carrier protein formation proceeds as follows. First, a prosthetic group precursor, presumably 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, is formed from ATP and dephospho-CoA in a reaction catalyzed by CitG. Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX. The conversion of apo-cyl carrier protein into holo-cyl carrier protein is achieved in vitro by incubation of apo-cyl carrier protein with proteins CitX, CitG, ATP, and dephospho-CoA. ATP cannot be substituted with GTP, CTP, UTP, ADP, or AMP. In the absence of CitG or dephospho-CoA, AMP-cyl carrier protein is formed. It is not possible to further convert AMP-cyl carrier protein to holo-cyl carrier protein by subsequent incubation with CitG and dephospho-CoA Klebsiella pneumoniae ?
-
?
additional information holo-acyl carrier protein formation proceeds as follows. First, a prosthetic group precursor, presumably 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, is formed from ATP and dephospho-CoA in a reaction catalyzed by CitG. Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX. The conversion of apo-cyl carrier protein into holo-cyl carrier protein is achieved in vitro by incubation of apo-cyl carrier protein with proteins CitX, CitG, ATP, and dephospho-CoA. ATP cannot be substituted with GTP, CTP, UTP, ADP, or AMP. In the absence of CitG or dephospho-CoA, AMP-cyl carrier protein is formed. It is not possible to further convert AMP-cyl carrier protein to holo-cyl carrier protein by subsequent incubation with CitG and dephospho-CoA Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? ? * 32600, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
CitG
-
Klebsiella pneumoniae
CitG
-
Escherichia coli