Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32600 | - |
? * 32600, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3'-dephospho-CoA | Escherichia coli | - |
2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain JM83, strain CM2080, gene citG | - |
Escherichia coli | P77231 | - |
- |
Klebsiella pneumoniae | P45414 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-4°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3'-dephospho-CoA | - |
Escherichia coli | 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine | - |
? | |
ATP + 3'-dephospho-CoA | ATP cannot be replaced by CTP, GTP or UTP | Escherichia coli | 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + adenine | - |
? | |
additional information | holo-acyl carrier protein formation proceeds as follows. First, a prosthetic group precursor, presumably 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, is formed from ATP and dephospho-CoA in a reaction catalyzed by CitG. Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX. The conversion of apo-cyl carrier protein into holo-cyl carrier protein is achieved in vitro by incubation of apo-cyl carrier protein with proteins CitX, CitG, ATP, and dephospho-CoA. ATP cannot be substituted with GTP, CTP, UTP, ADP, or AMP. In the absence of CitG or dephospho-CoA, AMP-cyl carrier protein is formed. It is not possible to further convert AMP-cyl carrier protein to holo-cyl carrier protein by subsequent incubation with CitG and dephospho-CoA | Klebsiella pneumoniae | ? | - |
? | |
additional information | holo-acyl carrier protein formation proceeds as follows. First, a prosthetic group precursor, presumably 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA, is formed from ATP and dephospho-CoA in a reaction catalyzed by CitG. Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX. The conversion of apo-cyl carrier protein into holo-cyl carrier protein is achieved in vitro by incubation of apo-cyl carrier protein with proteins CitX, CitG, ATP, and dephospho-CoA. ATP cannot be substituted with GTP, CTP, UTP, ADP, or AMP. In the absence of CitG or dephospho-CoA, AMP-cyl carrier protein is formed. It is not possible to further convert AMP-cyl carrier protein to holo-cyl carrier protein by subsequent incubation with CitG and dephospho-CoA | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 32600, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
CitG | - |
Klebsiella pneumoniae |
CitG | - |
Escherichia coli |