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Literature summary for 2.4.2.54 extracted from

  • Scott, J.W.; Rasche, M.E.
    Purification, overproduction, and partial characterization of beta-RFAP synthase, a key enzyme in the methanopterin biosynthesis pathway (2002), J. Bacteriol., 184, 4442-4448.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
2 * 32600, SDS-PAGE Methanosarcina thermophila
35500
-
2 * 35500, SDS-PAGE Archaeoglobus fulgidus
57000
-
gel filtration Archaeoglobus fulgidus
64500
-
gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate Archaeoglobus fulgidus the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate Methanosarcina thermophila the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28190
-
-
Methanosarcina thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila
partial Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.075
-
50°C, pH 5.0 Methanosarcina thermophila
0.29
-
50°C, pH 5.0 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Archaeoglobus fulgidus 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?
4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria Archaeoglobus fulgidus 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate
-
Methanosarcina thermophila 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria Methanosarcina thermophila 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32600, SDS-PAGE Methanosarcina thermophila
dimer 2 * 35500, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase
-
Methanosarcina thermophila
AF2089
-
Archaeoglobus fulgidus
beta-RFAP synthase
-
Methanosarcina thermophila
beta-RFAP synthase
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Methanosarcina thermophila
70
-
-
Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Methanosarcina thermophila
5.3
-
-
Archaeoglobus fulgidus