Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32600 | - |
2 * 32600, SDS-PAGE | Methanosarcina thermophila |
35500 | - |
2 * 35500, SDS-PAGE | Archaeoglobus fulgidus |
57000 | - |
gel filtration | Archaeoglobus fulgidus |
64500 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate | Archaeoglobus fulgidus | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | Methanosarcina thermophila | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28190 | - |
- |
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
partial | Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.075 | - |
50°C, pH 5.0 | Methanosarcina thermophila |
0.29 | - |
50°C, pH 5.0 | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Archaeoglobus fulgidus | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | Archaeoglobus fulgidus | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | - |
Methanosarcina thermophila | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | Methanosarcina thermophila | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32600, SDS-PAGE | Methanosarcina thermophila |
dimer | 2 * 35500, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase | - |
Methanosarcina thermophila |
AF2089 | - |
Archaeoglobus fulgidus |
beta-RFAP synthase | - |
Methanosarcina thermophila |
beta-RFAP synthase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Methanosarcina thermophila |
70 | - |
- |
Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Methanosarcina thermophila |
5.3 | - |
- |
Archaeoglobus fulgidus |