Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | the activity with CMP and UMP requires activation by cAMP | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
AMP | the kinetic parameters of the enzyme toward AMP are not determined, because kinetic equations that fit the data well can not be identified | Thermococcus kodakarensis | |
2.8 | - |
phosphate | pH 7.5, 85°C | Thermococcus kodakarensis | |
2.8 | - |
phosphate | pH 7.5, 85°C, cosubstrate: AMP | Thermococcus kodakarensis | |
4.4 | - |
UMP | pH 7.5, 85°C | Thermococcus kodakarensis | |
6.2 | - |
CMP | pH 7.5, 85°C | Thermococcus kodakarensis | |
18.5 | - |
GMP | pH 7.5, 85°C | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphate | Thermococcus kodakarensis | the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes | adenine + alpha-D-ribose 1,5-bisphosphate | - |
? | |
AMP + phosphate | Thermococcus kodakarensis | the enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes | adenine + alpha-D-ribose 1,5-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JCX3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermococcus kodakarensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate | (1) | Thermococcus kodakarensis | |
CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate | (2) | Thermococcus kodakarensis | |
UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate | (3) | Thermococcus kodakarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
substrate: GMP, pH 7.5, 85°C | Thermococcus kodakarensis |
10.8 | - |
substrate: UMP, pH 7.5, 85°C | Thermococcus kodakarensis |
15.9 | - |
substrate: AMP, pH 7.5, 85°C | Thermococcus kodakarensis |
37.5 | - |
substrate: CMP, pH 7.5, 85°C | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphate | - |
Thermococcus kodakarensis | adenine + alpha-D-ribose 1,5-bisphosphate | - |
? | |
AMP + phosphate | the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes | Thermococcus kodakarensis | adenine + alpha-D-ribose 1,5-bisphosphate | - |
? | |
AMP + phosphate | the enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes | Thermococcus kodakarensis | adenine + alpha-D-ribose 1,5-bisphosphate | - |
? | |
CMP + phosphate | - |
Thermococcus kodakarensis | cytosine + alpha-D-ribose 1,5-bisphosphate | - |
? | |
GMP + phosphate | - |
Thermococcus kodakarensis | guanine + alpha-D-ribose 1,5-bisphosphate | - |
? | |
additional information | the use of dAMP, dGMP, or dUMP results in the generation of only trace amounts of their corresponding nucleobases, adenine, guanine, or uracil, respectivelyno activity with: TMP, ADP, ATP, dCMP, dAMP, dGMP, dUMP | Thermococcus kodakarensis | ? | - |
? | |
UMP + phosphate | - |
Thermococcus kodakarensis | uracil + alpha-D-ribose 1,5-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMPpase | - |
Thermococcus kodakarensis |
deoA | - |
Thermococcus kodakarensis |
nucleoside monophosphate phosphorylase | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Thermococcus kodakarensis |
85 | - |
assay | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified | Thermococcus kodakarensis | |
additional information | - |
AMP | the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified | Thermococcus kodakarensis | |
2.7 | - |
GMP | pH 7.5, 85°C | Thermococcus kodakarensis | |
10.5 | - |
UMP | pH 7.5, 85°C | Thermococcus kodakarensis | |
15 | - |
phosphate | pH 7.5, 85°C | Thermococcus kodakarensis | |
15 | - |
phosphate | pH 7.5, 85°C, cosubstrate: AMP | Thermococcus kodakarensis | |
39.1 | - |
CMP | pH 7.5, 85°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified | Thermococcus kodakarensis | |
additional information | - |
AMP | the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified | Thermococcus kodakarensis | |
0.1 | - |
GMP | pH 7.5, 85°C | Thermococcus kodakarensis | |
2.4 | - |
UMP | pH 7.5, 85°C | Thermococcus kodakarensis | |
5.4 | - |
phosphate | pH 7.5, 85°C, cosubstrate: AMP | Thermococcus kodakarensis | |
6.3 | - |
CMP | pH 7.5, 85°C | Thermococcus kodakarensis |