Any feedback?
Literature summary for 2.4.2.7 extracted from
- Human adenine phosphoribosyltransferase. Purification, subunit structure, and substrate specificity (1973), J. Biol. Chem., 248, 2529-2535.
General Stability
| General Stability | Organism |
|---|---|
| ammonium sulfate, 1 M, partially protects against inactivation during storage | Homo sapiens |
| dithiothreitol, 10 mM, or dimethylsulfoxide, 5%, or 5-phospho-alpha-D-ribose 1-diphosphate, 1 mM, protects against inactivation during storage | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11100 | - |
3 * 11100, SDS-PAGE | Homo sapiens |
| 34000 | - |
gel filtration, sucrose density gradient ultracentrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.58 | - |
purified enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, purified enzyme, 5 mM MgCl2, 0.1 mM 5-phospho-alpha-D-ribose 1-diphosphate, stable for upt to 2 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 2,6-diaminopurine ribotide + diphosphate | - |
? | |
| 4-amino-5-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 4-amino-5-imidazolecarboxamide ribotide + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | best acceptor substrate | Homo sapiens | AMP + diphosphate | - |
? | |
| additional information | no donor substrates: D-ribose 5-phosphate, ribose 1-phosphate | Homo sapiens | ? | - |
? | |
| additional information | at 0°C in the absence of Mg2+ but in presence of substrates the enzyme catalyzes a rapid and limited synthesis of AMP | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 11100, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
at 0°C in the absence of Mg2+ but in presence of substrates enzyme catalyzes a rapid and limited synthesis of AMP | Homo sapiens |
| 37 | - |
assay at | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.5 | broad maximum | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
