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Literature summary for 2.4.2.7 extracted from
- Adenine phosphoribosyltransferase of Catharanthus roseus cells: purification, properties and regulation (1983), Z. Pflanzenphysiol., 110, 135-145.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Catharanthus roseus |  |
| AMP | competitive against 5-phospho-alpha-D-ribose 1-diphosphate | Catharanthus roseus | |
| GMP | - |
Catharanthus roseus | |
| Mg2+ | inhibition above 2 mM, activation below | Catharanthus roseus | |
| UMP | - |
Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
adenine | pH 7.6 | Catharanthus roseus | |
| 0.0096 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.6 | Catharanthus roseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Catharanthus roseus | |
| Mg2+ | optimal concentration: 2 mM | Catharanthus roseus | |
| Mg2+ | inhibition above 2 mM | Catharanthus roseus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Catharanthus roseus | adenine salvage enzyme | AMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.156 | - |
purified enzyme | Catharanthus roseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for at least 3 weeks | Catharanthus roseus |
| unstable at 4°C or at -20°C | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Catharanthus roseus | AMP + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | adenine salvage enzyme | Catharanthus roseus | AMP + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8 | - |
Catharanthus roseus |
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