Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Brassica juncea | |
p-hydroxymercuribenzoate | DTT | Brassica juncea | |
sulfhydryl reagents | - |
Brassica juncea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
adenine | - |
Brassica juncea | |
0.015 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Brassica juncea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Brassica juncea | |
Mg2+ | 3 mM at 60°C, at 37°C activity varies little in the range 3-50 mM MgCl2 | Brassica juncea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Brassica juncea |
54000 | - |
gel filtration | Brassica juncea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
cv. Green Wave | - |
Purification (Comment) | Organism |
---|---|
- |
Brassica juncea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica juncea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.15 | - |
- |
Brassica juncea |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 10 mM KCl, 10% glycerol, stable | Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Brassica juncea | AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27000, SDS-PAGE | Brassica juncea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brassica juncea |
60 | - |
- |
Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Brassica juncea |