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Literature summary for 2.4.2.7 extracted from

  • Phillips, C.L.; Ullman, B.; Brennan, R.G.; Hill, C.P.
    Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani (1999), EMBO J., 18, 3533-3545.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion method, hanging drops from solution: 10 mg/ml purified apo-enzyme in 10 mM MES, pH 6.0, 1 mM dithiothreitol, 5 mM MgCl2, 4°C, reservoir solution: 7-11% polyethylene glycol 5000 monomethyl ether, 0.2 M ammonium acetate, 0.1 M sodium citrate, pH 4.9, 10 mM MgCl2, 1.2-1.6 M ammonium sulfate, for AMP- or adenine-bound crystals addition of 10 mM AMP or 5 mM adenine in the reservoir solution, structure analysis Leishmania donovani

Protein Variants

Protein Variants Comment Organism
additional information alignment of amino acid sequences, correlation between human clinical missense mutations and structure, structure taken from Leishmania donovani enzyme Leishmania donovani
additional information alignment of amino acid sequences, correlation between human clinical missense mutations and structure, structure taken from Leishmania donovani enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Leishmania donovani
Mg2+ Mg2+ ligand binding site Leishmania donovani

Organism

Organism UniProt Comment Textmining
Homo sapiens P07741
-
-
Leishmania donovani Q27679
-
-

Reaction

Reaction Comment Organism Reaction ID
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate active site structure Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate substrate binding structure Leishmania donovani AMP + diphosphate AMP is bound in the low energy anti conformation with the ribose in the 2' endo conformation ?

Subunits

Subunits Comment Organism
dimer
-
Leishmania donovani
More three-dimensional structure, apo-enzyme in complexes with AMP, adenine, sulfate, citrate, subunit model Leishmania donovani