KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the 2 separated forms of enzyme in gel filtration without Mg2+ give 2 Km values for 5-phosphoribose 1-diphosphate | Saccharomyces cerevisiae | |
0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in presence of Mg2+ | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 20000 + 1 * 34000, SDS-PAGE | Saccharomyces cerevisiae |
34000 | - |
1 * 20000 + 1 * 34000, SDS-PAGE | Saccharomyces cerevisiae |
50000 | - |
gel filtration, in absence of Mg2+ the enzyme elutes as 2 distinct forms | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strain ATCC 24903 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants from Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.14 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 20000 + 1 * 34000, SDS-PAGE | Saccharomyces cerevisiae |