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Literature summary for 2.4.2.7 extracted from

  • Alfonzo-Garcia, J.; Sahota, A.; Taylor, M.W.
    Characterization of the adenine phosphoribosyltransferase from Saccharomyces cerevisiae (1994), Adv. Exp. Med. Biol., 370, 627-630.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the 2 separated forms of enzyme in gel filtration without Mg2+ give 2 Km values for 5-phosphoribose 1-diphosphate Saccharomyces cerevisiae
0.03
-
5-phospho-alpha-D-ribose 1-diphosphate in presence of Mg2+ Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
1 * 20000 + 1 * 34000, SDS-PAGE Saccharomyces cerevisiae
34000
-
1 * 20000 + 1 * 34000, SDS-PAGE Saccharomyces cerevisiae
50000
-
gel filtration, in absence of Mg2+ the enzyme elutes as 2 distinct forms Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
strain ATCC 24903
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutants from Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.14
-
purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Saccharomyces cerevisiae AMP + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 1 * 20000 + 1 * 34000, SDS-PAGE Saccharomyces cerevisiae