Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme in Escherichia coli strain BL21(DE3) and of the selenomethionine-labeled enzyme in Escherichia coli strain B834 | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant native and selenomethionine-labeled enzymes, sitting drop vapour diffusion method, 0.001 ml of 20 mg/ml native protein in 20 mM Tris-HCl, 50 mM NaCl, pH 8.0, is mixed with an equal volume of reservoir solution containing 100 mM MES, pH 5.5, 100 mM calcium acetate, 3% w/v PEG 10000, and 3% v/v MeOH, 1 week, for the selenomethionine-labeled protein 40 mM calcium acetate, 1.5% w/v PEG 10000, and 3% v/v MeOH is used at a protein concentration of 29.4 mg/ml, X-ray diffraction structure determination and analysis at 1.9-2.6 A resolution, multiple wavelength anomalous dispersion method, asymmetric unit of two pairs of identical dimers, each related by noncrystallographic two-fold symmetry, a fifth monomer forms a similar dimer across a crystallographic two-fold axis, modeling | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribosyl-1-diphosphate + adenine | Thermus thermophilus | the enzyme is involved in the salvage of adenosine, overview | AMP + diphosphate | - |
? | |
5-phospho-alpha-D-ribosyl-1-diphosphate + adenine | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme is involved in the salvage of adenosine, overview | AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) and recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834 by two steps of anion exchange chromatography, and gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribosyl-1-diphosphate + adenine | - |
Thermus thermophilus | AMP + diphosphate | - |
? | |
5-phospho-alpha-D-ribosyl-1-diphosphate + adenine | the enzyme is involved in the salvage of adenosine, overview | Thermus thermophilus | AMP + diphosphate | - |
? | |
5-phospho-alpha-D-ribosyl-1-diphosphate + adenine | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate | - |
? | |
5-phospho-alpha-D-ribosyl-1-diphosphate + adenine | the enzyme is involved in the salvage of adenosine, overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenine phosphoribosyltransferase | - |
Thermus thermophilus |
APRTase | - |
Thermus thermophilus |