Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D99N | mutant enzyme has very low activity | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | is a much stronger inhibitor than ATP at pH 4.5, but has virtually no effect on activity at neutral pH | Saccharolobus solfataricus | |
AMP | - |
Saccharolobus solfataricus | |
ATP | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24200 | - |
2 * 24200, SDS-PAGE | Saccharolobus solfataricus |
45000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | strong preference for adenine as substrate over the 6-oxopurine bases, hypoxanthine and guanine | Saccharolobus solfataricus | AMP + diphosphate | - |
? | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | strong preference for adenine as substrate over the 6-oxopurine bases, hypoxanthine and guanine | Saccharolobus solfataricus P2 | AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24200, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
adenione PRTase | - |
Saccharolobus solfataricus |
APRTase | - |
Saccharolobus solfataricus |
apT | - |
Saccharolobus solfataricus |
gpT-1 | - |
Saccharolobus solfataricus |
SSO2342 | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
the enzyme shows two pH optima, i.e., around 8 and 4.5. The activity of APRTase is considerably higher at pH 8 than pH 4.5, when assayed at 35°C, while the two activity peaks seems more equal, when assayed at 60°C | Saccharolobus solfataricus |
7.5 | 8.5 | the enzyme shows two pH optima, i.e., around 8 and 4.5. The activity of APRTase is considerably higher at pH 8 than pH 4.5, when assayed at 35°C, while the two activity peaks seems more equal, when assayed at 60°C | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
ADP | 60°C, pH 4.1 | Saccharolobus solfataricus | |
0.177 | - |
AMP | 60°C, pH 4.1 | Saccharolobus solfataricus | |
0.3 | - |
AMP | 60°C, pH 7.5 | Saccharolobus solfataricus | |
0.344 | - |
ATP | 60°C, pH 4.1 | Saccharolobus solfataricus | |
0.574 | - |
ATP | 60°C, pH 7.5 | Saccharolobus solfataricus | |
3.2 | - |
ADP | 60°C, pH 7.5 | Saccharolobus solfataricus |