General Stability | Organism |
---|---|
5-phospho-alpha-D-ribose 1-diphosphate stabilizes against heat inactivation | Streptomyces cyanogenus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptomyces cyanogenus | |
EDTA | - |
Streptomyces cyanogenus | |
GDP | - |
Streptomyces cyanogenus | |
GMP | - |
Streptomyces cyanogenus | |
GTP | - |
Streptomyces cyanogenus | |
IDP | - |
Streptomyces cyanogenus | |
IMP | - |
Streptomyces cyanogenus | |
purine nucleotides | - |
Streptomyces cyanogenus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
hypoxanthine | - |
Streptomyces cyanogenus | |
0.0027 | - |
guanine | - |
Streptomyces cyanogenus | |
0.063 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Streptomyces cyanogenus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activating effect of Mn2+ is higher than that of Mg2+ | Streptomyces cyanogenus | |
Mn2+ | activating effect of Mn2+ is higher than that of Mg2+ | Streptomyces cyanogenus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
8 * 18000, SDS-PAGE | Streptomyces cyanogenus |
150000 | - |
gel filtration | Streptomyces cyanogenus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cyanogenus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces cyanogenus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
66.5 | - |
purified enzyme | Streptomyces cyanogenus |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 7.8, 1 M KCl, 10 mM MgCl2, stable at least 1 month | Streptomyces cyanogenus |
4°C, 50 mM Tris-HCl, pH 7.8, complete loss of activity after 4 days | Streptomyces cyanogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Streptomyces cyanogenus | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Streptomyces cyanogenus | IMP + diphosphate | - |
? | |
additional information | no substrate: adenine | Streptomyces cyanogenus | ? | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | poor substrate | Streptomyces cyanogenus | XMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 18000, SDS-PAGE | Streptomyces cyanogenus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.4, stable | Streptomyces cyanogenus |
40 | - |
pH 7.4, gradual decrease of activity | Streptomyces cyanogenus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.4 | Streptomyces cyanogenus |
8.5 | - |
- |
Streptomyces cyanogenus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 9.7 | - |
Streptomyces cyanogenus |
6.3 | 9.7 | pH 6.3: about 50% of activity maximum, pH 9.7: about 75% of activity maximum | Streptomyces cyanogenus |