Any feedback?
Literature summary for 2.4.2.8 extracted from
- Chinese hamster hypoxanthine-guanine phosphoribosyltransferase. Purification, structural, and catalytic properties (1974), J. Biol. Chem., 249, 4030-4037.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | no inhibition | Cricetulus griseus |  |
| GMP | product inhibition | Cricetulus griseus | |
| IMP | product inhibition | Cricetulus griseus | |
| Inosine | slight inhibition | Cricetulus griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Cricetulus griseus | |
| 0.00052 | - |
hypoxanthine | - |
Cricetulus griseus | |
| 0.0011 | - |
guanine | - |
Cricetulus griseus | |
| 0.0053 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Cricetulus griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
3 * 25000, SDS-PAGE | Cricetulus griseus |
| 78000 | 85000 | gel filtration | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
at least 3 charge variant isoforms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from brain and liver | Cricetulus griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cricetulus griseus | - |
| liver | - |
Cricetulus griseus | - |
| V-79 cell | tissue culture cells | Cricetulus griseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9 | - |
purified enzyme, brain | Cricetulus griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Cricetulus griseus | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Cricetulus griseus | IMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 25000, SDS-PAGE | Cricetulus griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetulus griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
if first incubated in 1 mM 5-phospho-alpha-D-ribose 1-diphosphate, remarkably stable | Cricetulus griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Cricetulus griseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 11 | - |
Cricetulus griseus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
