Any feedback?
Literature summary for 2.4.2.8 extracted from
- Kinetic mechanism of human hypoxanthine-guanine phosphoribosyltransferase: rapid phosphoribosyl transfer chemistry (1997), Biochemistry, 36, 3700-3712.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for antiparasitic chemotherapy | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens | |
| 0.0024 | - |
hypoxanthine | recombinant enzyme | Homo sapiens |  |
| 0.0035 | - |
guanine | recombinant enzyme | Homo sapiens | |
| 0.0054 | - |
inosine monophosphate | recombinant enzyme | Homo sapiens | |
| 0.025 | - |
diphosphate | recombinant enzyme, with inosine monophosphate | Homo sapiens | |
| 0.035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, with hypoxanthine | Homo sapiens | |
| 0.065 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, with guanine | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
IMP + diphosphate | - |
? | |
| additional information | Homo sapiens | locus of Lesch-Nyhan syndrome, activator of the prodrugs 6-mercaptopurine and allopurinol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | kinetic mechanism | Homo sapiens | |
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | catalytic mechanism | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
kinetics | Homo sapiens |
| 0.57 | 0.76 | recombinant enzyme, reverse reaction | Homo sapiens |
| 21 | 23 | recombinant enzyme, forward reaction | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | guanine is utilized more rapidly than hypoxanthine | Homo sapiens | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | equilibrium lies far in direction of IMP formation | Homo sapiens | IMP + diphosphate | - |
r | |
| additional information | kinetic study | Homo sapiens | ? | - |
? | |
| additional information | locus of Lesch-Nyhan syndrome, activator of the prodrugs 6-mercaptopurine and allopurinol | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens | |
| 0.23 | 0.3 | inosine monophosphate | recombinant enzyme, reverse reaction | Homo sapiens | |
| 8.5 | 9.3 | hypoxanthine | recombinant enzyme | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
