Application | Comment | Organism |
---|---|---|
medicine | potential target for antiparasitic chemotherapy | Leishmania tarentolae |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli BL21(DE3) | Leishmania tarentolae |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme, 7 mg/ml, hanging-drop vapour-diffusion method, TMD buffer, pH 7.5, + equal volume of reservoir solution: 18°C or 4°C, pH 5.6 , 19% isopropanol, 19% polyethylene glycol 4000, 5% glycerol, or 17% polyethylene glycol 4000, 5% glycerol | Leishmania tarentolae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
guanine | recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania tarentolae | |
0.0044 | - |
hypoxanthine | recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania tarentolae | |
0.127 | - |
5-phosphoribosyl 1-diphosphate | recombinant enzyme, with guanine | Leishmania tarentolae | |
0.138 | - |
5-phosphoribosyl 1-diphosphate | recombinant enzyme, with hypoxanthine | Leishmania tarentolae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, recombinant enzyme, SDS-PAGE | Leishmania tarentolae |
50000 | - |
recombinant enzyme, gel filtration | Leishmania tarentolae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania tarentolae | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? | |
additional information | Leishmania tarentolae | enzyme is essential for salvaging exogenous purine bases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tarentolae | Q9NJI5 | gene hgprt | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Leishmania tarentolae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania tarentolae | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania tarentolae | IMP + diphosphate | - |
? | |
additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Leishmania tarentolae | ? | - |
? | |
additional information | enzyme is essential for salvaging exogenous purine bases | Leishmania tarentolae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, recombinant enzyme, SDS-PAGE | Leishmania tarentolae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme, pI: 8.2 | Leishmania tarentolae |