Any feedback?
Literature summary for 2.4.2.8 extracted from
- A non-active site mutation in human hypoxanthine guanine phosphoribosyltransferase expands substrate specificity (2004), Arch. Biochem. Biophys., 427, 116-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzyme in Escherichia coli, functional complementation study | Plasmodium falciparum |
| expression of wild-type and mutant enzymes in Escherichia coli, functional complementation study | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F36A | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
| F36E | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
| F36G | site-directed mutagenesis, inactive mutant | Homo sapiens |
| F36K | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
| F36L | random mutagenesis, mutant phosphoribosylates xanthine, mutant does not bind the purine substrate directly, long-range modulation via loop IV influence the substrate specificity, altered enzyme stability | Homo sapiens |
| F36W | site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme | Homo sapiens |
| L44F | site-directed mutagenesis, mutant does not phosphoribosylate guanine and xanthine, modulation via loop IV influence the substrate specificity, altered enzyme stability | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36L, with xanthine | Homo sapiens |  |
| 0.001 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
| 0.001 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme and mutant L44F | Plasmodium falciparum | |
| 0.0014 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.0016 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
| 0.0018 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
| 0.002 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
| 0.002 | - |
guanine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
| 0.0029 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
| 0.0044 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36K, with hypoxanthine | Homo sapiens | |
| 0.0044 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
| 0.0045 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.0061 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
| 0.011 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36G, with hypoxanthine | Homo sapiens | |
| 0.027 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36L, with hypoxanthine | Homo sapiens | |
| 0.029 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36K, with guanine | Homo sapiens | |
| 0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine | Plasmodium falciparum | |
| 0.035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine | Homo sapiens | |
| 0.041 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36L, with guanine | Homo sapiens | |
| 0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant F36G, with guanine | Homo sapiens | |
| 0.076 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with guanine | Homo sapiens | |
| 0.079 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with xanthine | Plasmodium falciparum | |
| 0.102 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant L44F, with hypoxanthine | Plasmodium falciparum | |
| 0.116 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant L44F, with guanine | Plasmodium falciparum | |
| 0.151 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant mutant L44F, with xanthine | Plasmodium falciparum | |
| 0.201 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 22°C, recombinant wild-type enzyme, with guanine | Plasmodium falciparum | |
| 0.261 | - |
xanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
| 0.287 | - |
xanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
| 0.853 | - |
xanthine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GMP + diphosphate | Homo sapiens | - |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
IMP + diphosphate | - |
? | |
| IMP + diphosphate | Homo sapiens | - |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | wild-type enzyme | xanthosine 5'-phosphate + diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | mutant F36L, no activity with the wild-type enzyme | xanthosine 5'-phosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00492 | - |
- |
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant L44F from Escherichia coli | Plasmodium falciparum |
| recombinant wild-type and mutants F36L, F36G, F36K from Escherichia coli | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | residue F36 is responsible for substrate specificity, structure-function relationship | Homo sapiens | |
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | residue L44 is responsible for substrate specificity, structure-function relationship | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GMP + diphosphate | - |
Homo sapiens | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme, no activity with mutant F36L | Homo sapiens | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme, no activity with mutant F36L | Homo sapiens | IMP + diphosphate | - |
? | |
| IMP + diphosphate | - |
Homo sapiens | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| additional information | the flexibilty of loop IV can influence the substrate specificity | Homo sapiens | ? | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Plasmodium falciparum | xanthosine 5'-phosphate + diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | mutant F36L, no activity with the wild-type enzyme | Homo sapiens | xanthosine 5'-phosphate + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGPRT | - |
Plasmodium falciparum |
| HGPRT | - |
Homo sapiens |
| hypoxanthine guanine phosphoribosyltransferase | - |
Plasmodium falciparum |
| hypoxanthine guanine phosphoribosyltransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Plasmodium falciparum |
| 22 | - |
assay at room temperature | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
3 h, wild-type and mutant F36L, over 80% remaining activity | Homo sapiens |
| 70 | - |
0.5 h, wild-type and mutant F36L, over 80% remaining activity | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
xanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
| 0.94 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
| 1.1 | - |
guanine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
| 1.13 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
| 1.2 | - |
xanthine | pH 7.4, 22°C, recombinant mutant L44F | Plasmodium falciparum | |
| 1.2 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
| 2.9 | - |
xanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Plasmodium falciparum | |
| 3.1 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
| 3.4 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36G | Homo sapiens | |
| 3.4 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
| 5.4 | - |
hypoxanthine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
| 6.3 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36K | Homo sapiens | |
| 7.1 | - |
hypoxanthine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
| 18 | - |
guanine | pH 7.4, 22°C, recombinant mutant F36L | Homo sapiens | |
| 25.5 | - |
guanine | pH 7.4, 22°C, recombinant wild-type enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Plasmodium falciparum |
| 7.4 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
