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Literature summary for 2.4.2.8 extracted from

  • Argyros, D.A.; Tripathi, S.A.; Barrett, T.F.; Rogers, S.R.; Feinberg, L.F.; Olson, D.G.; Foden, J.M.; Miller, B.B.; Lynd, L.R.; Hogsett, D.A.; Caiazza, N.C.
    High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes (2011), Appl. Environ. Microbiol., 77, 8288-8294.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology genetic tools for use in Clostridium thermocellum that allow creation of unmarked mutations while using a replicating plasmid. The strategy employs counter-selections developed from the native Clostridium thermocellum hpt gene and the Thermoanaerobacterium saccharolyticum tdk gene and is used to delete the genes for both lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta) Acetivibrio thermocellus
synthesis a lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta) deletion strain is evolved for 2,000 h, resulting in a stable strain with 40:1 ethanol selectivity and a 4.2-fold increase in ethanol yield over the wild-type strain. In a coculture of organic acid-deficient engineered strains of both Clostridium thermocellum and Thermoanaerobacterium saccharolyticum, fermentation of 92 g/liter Avicel results in 38 g/liter ethanol, with acetic and lactic acids below detection limits, in 146 h. engineering is based on a phosphoribosyl transferase (Hpt) deletion strain, which produces acetate, lactate, and ethanol in a ratio of 1.7:1.5:1.0, similar to the 2.1:1.9:1.0 ratio produced by the wild type. The Hpt/Ldh double mutant strain does not produce significant levels of lactate and has a 1.4:1.0 ratio of acetate to ethanol. Similarly, the Hpt/Pta double mutant strain does not produce acetate and has a 1.9:1.0 ratio of lactate to ethanol. The Hpt/Ldh/Pta triple mutant strain achieves ethanol selectivity of 40:1 relative to organic acids Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DHM7
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Acetivibrio thermocellus DSM 1237 A3DHM7
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Synonyms

Synonyms Comment Organism
Cthe_2254
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Acetivibrio thermocellus
hypoxanthine phosphoribosyltransferase
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Acetivibrio thermocellus