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Literature summary for 2.4.2.8 extracted from
- Oligomeric state of hypoxanthine-guanine phosphoribosyltransferase from Mycobacterium tuberculosis (2017), Biochimie, 135, 6-14 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | the tetrameric enzyme activity increases in phosphate buffer, while the dimeric state, which occurs when excess magnesium ions are removed, is unable to be rapidly activated by phosphate and magnesium. Phosphate alone cannot activate the enzyme, magnesium ions are also required | Mycobacterium tuberculosis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-((2-(guanin-9-yl)ethyl)(2-((2-hydroxyethyl)((2-phosphonoethyl)amino)ethyl)amino)ethyl)phosphonic acid | - |
Mycobacterium tuberculosis | |
| 2-(2-((2-(hypoxanthine-9-yl)ethyl)((2-phosphonoethyl)amino)ethyl)((2-phosphonoethyl)amino)ethyl)phosphonic acid | - |
Mycobacterium tuberculosis | |
| 2-([3-(guanin-9-yl)-2-(2-bis(hydroxyphosphoryl)ethoxy)propoxy]ethyl)phosphonic acid | - |
Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on. Phosphate alone cannot activate the enzyme, magnesium ions are also required | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45400 | - |
dimeric enzyme, sedimentation equilibrium | Mycobacterium tuberculosis |
| 72600 | - |
enzyme in dimer-tetramer equilibrium, gel filtration | Mycobacterium tuberculosis |
| 96000 | - |
tetrameric enzyme, gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
GMP + diphosphate | - |
r | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
GMP + diphosphate | - |
r | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis ATCC 25618 | - |
GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis ATCC 25618 | - |
IMP + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WHQ9 | - |
- |
| Mycobacterium tuberculosis ATCC 25618 | P9WHQ9 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WHQ9 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2524 | - |
purified enzyme in phosphate buffer, pH 7.4, 25°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | GMP + diphosphate | - |
r | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | GMP + diphosphate | - |
r | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis ATCC 25618 | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis ATCC 25618 | IMP + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mycobacterium tuberculosis |
| More | dimer-tetramer equilibrium, the major peak is in a dimeric state. DTT is absent from the buffers containing MtHGPRT as there is only one cysteine residue per subunit and this enzyme does not undergo oxidation in the absence of reducing agents. MtHGPRT elutes as three different oligomeric species. MtHGPRT in the Tris-chloride-Mg2+ comprises a mixture of oligomeric states coexisting in a self-association equilibrium. Both tetrameric and dimeric forms of the enzyme are active after column chromatography. The specific activity of the tetramer appears higher than that of the dimer. Crystals of enzyme-diphosphate-GMP complex are used for structure determination, detailed overview | Mycobacterium tuberculosis |
| tetramer | - |
Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hypoxanthine-guanine phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
| MtHGPRT | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mycobacterium tuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | crystals of enzyme-diphosphate-GMP complex are used for structure determination, detailed overview | Mycobacterium tuberculosis |
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