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Literature summary for 2.4.2.9 extracted from
- Nucleotide synthesis from 5-phosphoribosylpyrophosphate (1963), Methods Enzymol., 6, 136-158.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | omission of glutathione reduces the reaction rate about 25% | Lactobacillus bifidus |  |
General Stability
| General Stability | Organism |
|---|---|
| dialysis against buffers of low ionic strength invariably results in complete inactivation, whereas after 3 h dialysis against 0.5 M KCl only 40% of the activity is lost | Lactobacillus bifidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | 80% inhibition at 0.05 M | Lactobacillus delbrueckii subsp. bulgaricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Lactobacillus bifidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Lactobacillus bifidus | |
| Mg2+ | only 5% of the activity remaining in absence | Lactobacillus bifidus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strain ATCC 9637 | - |
| Escherichia coli | - |
not: Escherichia coli B | - |
| Lactobacillus bifidus | - |
strain ATCC 4963 | - |
| Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
| Lactobacillus delbrueckii subsp. bulgaricus 09X | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus bifidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.025 | - |
purified enzyme | Lactobacillus bifidus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, partially purified enzyme, loss of 10% activity within 2 weeks | Lactobacillus bifidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 5-fluoro-UMP + diphosphate | - |
r | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | orotic acid is no substrate | Lactobacillus bifidus | ? | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Lactobacillus delbrueckii subsp. bulgaricus | UMP + diphosphate | - |
r | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Lactobacillus bifidus | UMP + diphosphate | - |
r | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | equilibrium lies far in the direction of UMP formation | Escherichia coli | UMP + diphosphate | - |
r | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Lactobacillus delbrueckii subsp. bulgaricus 09X | UMP + diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Lactobacillus bifidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Lactobacillus bifidus |
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