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Literature summary for 2.4.2.9 extracted from

  • Lindsay, R.H.; Tillery, C.R.; Yu, M.Y.W.
    Conversion of the antithyroid drug 2-thiouracil to 2-thio-5-UMP by UMP pyrophosphorylase (1972), Arch. Biochem. Biophys., 148, 466-474.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
K+ slight inhibition at 0.1 M Lactobacillus leichmannii
Na+ slight inhibition at 0.1 M Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
Uracil
-
Lactobacillus leichmannii
0.67
-
2-Thiouracil
-
Lactobacillus leichmannii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Lactobacillus leichmannii

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
-
strain ATCC 4797
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0057
-
substrate 2-thiouracil Lactobacillus leichmannii
0.0067
-
substrate uracil Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-thiouracil + 5-phospho-alpha-D-ribose 1-diphosphate substrate is anti-thyroid drug Lactobacillus leichmannii 2-thio-5'-UMP + diphosphate
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
Lactobacillus leichmannii UMP + diphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Lactobacillus leichmannii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 plateau, substrate uracil Lactobacillus leichmannii
7.5 8 substrate 2-thiouracil Lactobacillus leichmannii
7.8
-
2-thiouracil Lactobacillus leichmannii