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Literature summary for 2.4.2.9 extracted from

  • Islam, M.R.; Kim, H.; Kang, S.W.; Kim, J.S.; Jeong, Y.M.; Hwang, H.J.; Lee, S.Y.; Woo, J.C.; Kim, S.G.
    Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana (2007), Plant Mol. Biol., 63, 465-477.
    View publication on PubMed
Search for more literature for 2.4.2.9

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of the natural chimeric gene UK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT, expressionin Escherichia coli, transient expression as C-terminally GFP-tagged enzyme in Arabidopsis thaliana mesophyll protoplasts, 5-FU sensitivity of the Escherichia coli upp mutants is influenced by the expression of AtUK/UPRT1, as well as the 5-FU and 5-FD sensitivity of Escherichia coli upp-udk mutants, overview Arabidopsis thaliana
expressed in Escherichia coli strain JM109 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm forming granular precipitates Arabidopsis thaliana 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, recombinant UK/UPRT1, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate Arabidopsis thaliana recombinant bifunctional enzyme UK/UPRT 5-fluoro-UMP + diphosphate
-
 ?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate Arabidopsis thaliana
-
 UMP + diphosphate
-
 ?
uridine + ATP Arabidopsis thaliana the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48 UMP + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FKS0 ecotype Col-0
-
Arabidopsis thaliana Q9FKS0 AtUK/UPRT1; ecotype Col-0, several strains, natural chimeric gene AtUK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
flower lower expression level Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
leaf higher expression level Arabidopsis thaliana
-
additional information ubiquitous expression of UK/UPRT Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
root lower expression level Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-
stem higher expression level Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana 5-fluoro-UMP + diphosphate
-
 ?
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate recombinant bifunctional enzyme UK/UPRT Arabidopsis thaliana 5-fluoro-UMP + diphosphate
-
 ?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana UMP + diphosphate
-
 ?
uridine + ATP the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48 Arabidopsis thaliana UMP + ADP
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, recombinant UK/UPRT1, SDS-PAGE Arabidopsis thaliana
More the recombinant chimeric enzyme UK/UPRT1 shows an N-terminal region containing a UK domain and the C-terminal region consisting of an UPRT domain Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
More cf. EC 2.7.1.48 Arabidopsis thaliana
UK/UPRT1
-
 Arabidopsis thaliana
UPRT
-
 Arabidopsis thaliana
uridine kinase-like protein (uracil phosphoribosyltransferase)
-
 Arabidopsis thaliana
uridine kinase/uracil phosphoribosyltransferase 1 the enzyme contains a uridine domain and the C-terminal region consists of a UPRT domain Arabidopsis thaliana