KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
N-benzoyl-Asn-Gly-Thr-NHCH3 | - |
Sus scrofa | |
0.6 | - |
N-benzoyl-Asn-Gly-Ser-NHCH3 | - |
Sus scrofa | |
4.3 | - |
N-benzoyl-Asn-Gly-L-threo-beta-hydroxynorvaline-NHCH3 | - |
Sus scrofa | |
12.5 | - |
N-benzoyl-Asn-Gly-D-allo-Thr-NHCH3 | - |
Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | Sus scrofa | N-glycosylation of eukaryotic secretory and membrane-bound proteins is an essential and highly conserved protein modification. The key step in this pathway is the en bloc transfer of the high mannose core oligosaccharide Gly3Man9GlcNAc2 from the lipid carries dolichyl phosphate to selected Asn-X-Ser/Thr sequences of nascent polypeptide chains during their translocation across the endoplasmic reticulum membrane | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl diphosphooligosaccharide + N-benzoyl-Asn-Gly-D-allo-Thr-NHCH3 | - |
Sus scrofa | ? | - |
? | |
dolichyl diphosphooligosaccharide + N-benzoyl-Asn-Gly-L-threo-beta-hydroxynorvaline-NHCH3 | - |
Sus scrofa | ? | - |
? | |
dolichyl diphosphooligosaccharide + N-benzoyl-Asn-Gly-Ser-NHCH3 | - |
Sus scrofa | ? | - |
? | |
dolichyl diphosphooligosaccharide + protein L-asparagine | highly stereospecific for the conformation of the 3-carbon atom in the hydroxy amino acid. Binding of the threonine beta-methyl group by the enzyme is also specific, with serine, L-threo-beta-hydroxynorvaline and L-beta-hydroxynorleucine containing tripeptides all bound less efficiently than the threonine CH3-CH(OH) group | Sus scrofa | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
dolichyl-diphosphochitobiose + N-benzoyl-Asn-Gly-Thr-NHCH3 | - |
Sus scrofa | ? | - |
? | |
lipid-linked oligosaccharide + unfolded nascent polypeptide chain | N-glycosylation of eukaryotic secretory and membrane-bound proteins is an essential and highly conserved protein modification. The key step in this pathway is the en bloc transfer of the high mannose core oligosaccharide Gly3Man9GlcNAc2 from the lipid carries dolichyl phosphate to selected Asn-X-Ser/Thr sequences of nascent polypeptide chains during their translocation across the endoplasmic reticulum membrane | Sus scrofa | ? | - |
? |