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Literature summary for 2.4.99.18 extracted from
- Heterologous expression and biophysical characterization of soluble oligosaccharyl transferase subunits (2004), Arch. Biochem. Biophys., 431, 63-70.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the large soluble domains of the subunits Nlt1p, Swp1p and Wbp1p are expressed in a baculovirus-infected insect cell system | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | oligosaccharyl transferase catalyzes the first committed step in N-linked protein glycosylation, a cotranslational process that occurs in the lumen of the endoplasmic reticulum | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the large soluble domains of the subunits Nlt1p and Wbp1p are glycosylated | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | oligosaccharyl transferase catalyzes the first committed step in N-linked protein glycosylation, a cotranslational process that occurs in the lumen of the endoplasmic reticulum | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | multimeric membrane-bound enzyme complex comprising nine integral membrane subunits | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| oligosaccharyl transferase | - |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 54 | - |
absolute melting temperature of large soluble domain of the subunit Nlt1p | Saccharomyces cerevisiae |
| 65 | - |
absolute melting temperature of large soluble domain of the subunit Swp1p | Saccharomyces cerevisiae |
| 73 | - |
absolute melting temperature of large soluble domain of the subunit Wbp1p | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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