Literature summary for 2.4.99.18 extracted from

Li, G.; Yan, Q.; Nita-Lazar, A.; Haltiwanger, R.S.; Lennarz, W.J.
Studies on the N-glycosylation of the subunits of oligosaccharyl transferase in Saccharomyces cerevisiae (2005), J. Biol. Chem., 280, 1864-1871.

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Protein Variants

Protein Variants	Comment	Organism
N217Q	mutant of subunit Ost1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N332Q	mutant of subunit Wbp1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N336Q	mutant of subunit Ost1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N400Q	mutant of subunit Ost1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N535Q	mutant of subunit Stt3p, lethal phenotype, glycosylation change detected	Saccharomyces cerevisiae
N536Q	mutant of subunit Stt3p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N539Q	mutant of subunit Stt3p, lethal phenotype, glycosylation change detected	Saccharomyces cerevisiae
N540Q	mutant of subunit Stt3p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N60Q	mutant of subunit Stt3p, mutant of subunit Stt3p, normal growth phenotype, no glycosylation change detected	Saccharomyces cerevisiae
N60Q	mutant of subunit Wbp1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N99Q	mutant of subunit Ost1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
N99Q/N217Q	mutant of subunit Ost1p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
T537A	mutant of subunit Stt3p, lethal phenotype, glycosylation change detected	Saccharomyces cerevisiae
T537A/N539Q	mutant of subunit Stt3p, lethal phenotype, glycosylation change detected	Saccharomyces cerevisiae
T537S	mutant of subunit Stt3p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae
T541A	mutant of subunit Stt3p, extremely temperature-sensitive phenotype, glycosylation change detected	Saccharomyces cerevisiae
T541S	mutant of subunit Stt3p, normal growth phenotype, glycosylation change detected	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P33767	WBP1 beta-subunit	-
Saccharomyces cerevisiae	P39007	STT3	-
Saccharomyces cerevisiae	P41543	alpha-subunit OST1	-
Saccharomyces cerevisiae	P46964	epsilon-subunit OST2	-
Saccharomyces cerevisiae	P48439	gamma-subunit OST3	-
Saccharomyces cerevisiae	Q02795	SWP1 delta-subunit	-
Saccharomyces cerevisiae	Q03723	OST6	-
Saccharomyces cerevisiae	Q92316	OST5	-
Saccharomyces cerevisiae	Q99380	OST4	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	oligosaccharyl transferase consists of nine different subunits. Three of the essential gene products, Ost1p, Wbp1p, and Stt3p, are N-linked glycoproteins. The four potential N-glycosylation sites in Ost1p and the two potential N-glycosylation sites in Wbp1p are occupied in the mature proteins. None of the N-glycosylation sites in these two proteins is conserved, and no defect in growth or oligosaccharyl transferase activity is observed when the N-glycosylation sites are mutated to block N-glycosylation in either subunit. Glycan chain of the Stt3p subunit (Man8GlcNAc2) is linked only to Asn539	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
oligosaccharyl transferase	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)