Cloned (Comment) | Organism |
---|---|
PglB is overexpressed in Escherichia coli C43(DE3) at a level of 1 mg/L cell cultures | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | PglB possesses 11 transmembrane segments and two relatively large periplasmic regions other than the C-terminal domain | Campylobacter jejuni | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q9S4V7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentaprenyl diphospho-N-acetyl-D-galactosamine + [protein]-L-asparagine | PglB can utilize shorter polyisoprenol (pentaprenol) as the lipid carrier, albeit with reduced efficiency | Campylobacter jejuni | pentaprenyl diphosphate + [protein]-N-(N-acetyl-D-galactosaminyl)-L-asparagine | - |
? | |
undecaprenyl diphospho-N-acetyl-D-galactosamine + [protein]-L-asparagine | PglB is solely responsible for the oligosaccharyltransferase activity. PglB can transfer a monosaccharide, e.g. GalNAc to a peptide acceptor. PglB exhibits relaxed sugar substrate specificity | Campylobacter jejuni | undecaprenyl diphosphate + [protein]-N-(N-acetyl-D-galactosaminyl)-L-asparagine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PglB | - |
Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Campylobacter jejuni |