Application | Comment | Organism |
---|---|---|
medicine | enzyme inhibition could have therapeutic potential against trypanosomiasis | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
PCR-amplification, TbSTT3A expression in Saccharomyces cerevisiae does not replace yeast SST3 | Trypanosoma brucei |
PCR-amplification, TbSTT3B expression in Saccharomyces cerevisiae can replace yeast SST3, mild temperature-sensitive growth at 37°C, wild-type growth at 23°C | Trypanosoma brucei |
PCR-amplification, TbSTT3C expression in Saccharomyces cerevisiae can replace yeast SST3, severe growth defects at 23°C | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNAi | silencing of TbSTT3A | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl diphosphooligosaccharide + protein L-asparagine | Trypanosoma brucei | - |
dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | TbSTT3A transcribed in the bloodstream nor procyclic form | Trypanosoma brucei | - |
additional information | TbSTT3B transcribed in the bloodstream nor procyclic form | Trypanosoma brucei | - |
additional information | TbSTT3C not transcribed in the bloodstream nor procyclic form | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biantennary Man5GlcNAc2 dolichyl disphosphate + VSG221 coat glycoprotein | selective transfer of TbSTT3A to acidic to neutral regions of polypeptides, responsible for all paucimannose and complex N-glycans in Trypanosoma brucei glycoproteins | Trypanosoma brucei | dolichyl disphosphate + ? | - |
? | |
biantennary Man5GlcNAc2 dolichyl disphosphate + VSG221 coat glycoprotein | selective transfer of TbSTT3A to acidic to neutral regions of polypeptides, responsible for all paucimannose and complex N-glycans in Trypanosoma brucei glycoproteins | Trypanosoma brucei | dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine | - |
? | |
dolichyl diphosphooligosaccharide + protein L-asparagine | - |
Trypanosoma brucei | dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine | - |
? | |
triantennary Man9GlcNAc2 dolichyl disphosphate + VSG221 coat glycoprotein | selective transfer of TbSTT3B to neutral to basic regions of polypeptides responsible for most or all oligomannose N-glycans in Trypanosoma brucei glycoproteins | Trypanosoma brucei | dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oligosaccharyltransferase | - |
Trypanosoma brucei |
OTase | - |
Trypanosoma brucei |
TbSTT3A | - |
Trypanosoma brucei |
TbSTT3B | - |
Trypanosoma brucei |
TbSTT3C | - |
Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | simultaneous TbSTT3A and TbSTT3B knockdown prevents parasite growth in the bloodstream | Trypanosoma brucei |
physiological function | TbSTT3A and TbSTT3B N-glycosylation together is essential for infectivity in mice | Trypanosoma brucei |