Literature summary for 2.4.99.18 extracted from

Nasab, F.P.; Schulz, B.L.; Gamarro, F.; Parodi, A.J.; Aebi, M.
All in one: Leishmania major STT3 proteins substitute for the whole oligosaccharyltransferase complex in Saccharomyces cerevisiae (2008), Mol. Biol. Cell, 19, 3758-3768.

Search for more literature for 2.4.99.18

Cloned(Commentary)

Cloned (Comment)	Organism
Lm STT3 paralogues LmSTT3A, LmSTT3B, LmSTT3C, or LmSTT3D are expressed individually in Saccharomyces cerevisiae. Three complemented the yeast stt3 deletion. LmSTT3D expression suppresses deletions of other essential OTase subunits (Wbp1p, Ost1p, Ost2p, and Swp1p). Leishmania major STT3 paralogues are active enzymes that do not incorporate into the yeast OTase complex, but instead form dimers	Leishmania major

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	2 * 80000, SDS-PAGE	Leishmania major
550000	-	non-denaturing PAGE	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dolichyl diphosphooligosaccharide + [protein]-L-asparagine	Leishmania major	-	dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	-	-	-
Saccharomyces cerevisiae	P33767 and Q02791 and P39007 and P41543 and P48439	the enzyme consists of nine subunits. Five of them, Wbp1, Swp1, Stt3, Ost1, and Ost2, are essential for viability of the cell, whereas Ost4, Ost5, Ost3, and Ost6 are not essential but are required for maximal OST activity: Wbp1 (P33767 (SwissProt)), Swp1 (Q02795 (SwissProt)), Stt3 (P39007 (SwissProt)), Ost1 (P41543 (SwissProt)), Ost2 (P46964 (SwissProt)), Ost3 (P48439 (SwissProt)), Ost4 (Q99380 (SwissProt)), Ost5, and Ost6	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dolichyl diphosphooligosaccharide + [protein]-L-asparagine	-	Leishmania major	dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine	-	?
dolichyl diphosphooligosaccharide + [protein]-L-asparagine	various LmOTases have different protein substrate affinities when expressed in yeast. Biosynthetic intermediates as well as the complete Glc3Man9GlcNAc2 oligosaccharide are transferred efficiently to protein by the LmSTT3D OTase isoform without any apparent substrate specificity	Leishmania major	dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 80000, SDS-PAGE	Leishmania major

Synonyms

Synonyms	Comment	Organism
Lm STT3	-	Leishmania major
oligosaccharyltransferase complex	-	Saccharomyces cerevisiae
STT3 protein	-	Leishmania major

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Release 2023.1 (January 2023)