Literature summary for 2.4.99.19 extracted from

Wacker, M.; Feldman, M.F.; Callewaert, N.; Kowarik, M.; Clarke, B.R.; Pohl, N.L.; Hernandez, M.; Vines, E.D.; Valvano, M.A.; Whitfield, C.; Aebi, M.
Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems (2006), Proc. Natl. Acad. Sci. USA, 103, 7088-7093.

Search for more literature for 2.4.99.19

Cloned(Commentary)

Cloned (Comment)	Organism
gene pglB, functional expression in Escherichia coli strains E69, CWG28, or CWG44 using an arabinose-inducible promoter, coexpression of the Acra protein substrate	Campylobacter jejuni

Protein Variants

Protein Variants	Comment	Organism
additional information	functional transfer of the Campyobacter jejuni glycosylation system into Salmonella enterica	Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine	Campylobacter jejuni	-	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	-	gene pglB	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the PglB relaxed oligosaccharide substrate specificity allows the transfer of different glycans from the lipid carrier undecaprenyl pyrophosphate to an acceptor protein, substrate specificity of PglB with a set of lipid-linked polysaccharides in Escherichia coli and Salmonella enterica serovar Typhimurium. PglB required an acetamido group at the C-2, while a hexose linked to the C-6 of the monosaccharide at the reducing end does not inhibit the transfer of the O antigen to the acceptor protein, mechanism of PglB, modeling, overview. The Salmonella enterica O antigen containing Gal at the reducing end , and K30 capsular antigen, and LT2 antigen are not substrates for PglB, as	Campylobacter jejuni	?	-	?
tritrans,heptacis-undecaprenyl diphospho-O1 antigen + [protein]-L-asparagine	from Shigella dysenteriae	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O1 antigen	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?
tritrans,heptacis-undecaprenyl diphospho-O11 antigen + [protein]-L-asparagine	from Pseudomonas aeruginosa	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O11 antigen	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?
tritrans,heptacis-undecaprenyl diphospho-O157 antigen + [protein]-L-asparagine	from Escherichia coli	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O157 antigen	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?
tritrans,heptacis-undecaprenyl diphospho-O16 antigen + [protein]-L-asparagine	from Escherichia coli	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O16 antigen	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?
tritrans,heptacis-undecaprenyl diphospho-O7 antigen + [protein]-L-asparagine	from Escherichia coli	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O7 antigen	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?
tritrans,heptacis-undecaprenyl diphospho-O9 antigen + [Acra]-L-asparagine	PglB transfers the O9a antigen, an ABC transporter-dependent O antigen, to AcrA in Escherichia coli strain CWG28, the K30 antigen is not transferred to AcrA	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [Acra]-L-asparagine-O9 antigen	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine	-	Campylobacter jejuni	tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide	a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine	?

Synonyms

Synonyms	Comment	Organism
bacterial oligosaccharyltransferase	-	Campylobacter jejuni
oligosacharyltransferase	-	Campylobacter jejuni
OTase	-	Campylobacter jejuni
PglB	-	Campylobacter jejuni

General Information

General Information	Comment	Organism
evolution	eukaryotic and prokaryotic OTases catalyze the transfer of oligosaccharides by a conserved mechanism	Campylobacter jejuni

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)