Cloned (Comment) | Organism |
---|---|
gene pglB, sequence comparisons and phylogenetic analysis, cloning and analysis of an operon that might encode all enzymes required for N-glycosylation in Desulfovibrio desulfuricans, functional expression of gene pglB in Eschericia coli | Desulfovibrio desulfuricans |
gene pglB, sequence comparisons and phylogenetic analysis, functional expression in Escherichia coli. All genes necessary for N-linked glycosylation in Campylobacter jejuni are located in a single locus | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Desulfovibrio desulfuricans | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene pglB | - |
Desulfovibrio desulfuricans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PglBCj adds two glycan chains to model protein acceptor AcrA. PglBCj recognizes the conventional bacterial glycosylation sequon consisting of the sequence D/E-X1-NNX2-S/T, where X1 and X2 are any amino acid except proline, in contrast to PglBDd from Desulfovibrio desulfuricans, overview. AcrA contains five potential N-glycosylation sites N-X-S/T, but only the two that have an Asp residue at the -2 position are recognized by the Campylobacter jejuni enzyme, N123 and N273 | Campylobacter jejuni | ? | - |
? | |
additional information | the recombinantly expressed PglB can transfer the Campylobacter jejuni heptasaccharide onto the model protein acceptor AcrA, PglBDd adds a single glycan chain to AcrA. PglBDd does not recognize the conventional bacterial glycosylation sequon consisting of the sequence D/E-X1-NNX2-S/T, where X1 and X2 are any amino acid except proline, and instead uses a different site for the attachment of the oligosaccharide than PglBCj from Campylobacter jejuni, PglBDd exhibits relaxed glycan specificity, being able to transfer mono- and polysaccharides to AcrA, overview | Desulfovibrio desulfuricans | ? | - |
? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [HmcA]-L-asparagine | a protein from Desulfovibrio gigas, N-glycosylation site is N261 | Desulfovibrio desulfuricans | tritrans,heptacis-undecaprenyl diphosphate + [HmcA]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Desulfovibrio desulfuricans | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | PglBDd can transfer mono- and polysaccharides. LLO and AcrA and substrate glycosylation sites, overview. PglBDd does not require a negatively charged residue in the -2 position of the glycosylation sequon N-X-S/T | Desulfovibrio desulfuricans | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
Subunits | Comment | Organism |
---|---|---|
More | STT3 catalytic subunit sequence comparisons, overview | Desulfovibrio desulfuricans |
More | STT3 catalytic subunit sequence comparisons, overview | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
N-OTase | - |
Desulfovibrio desulfuricans |
N-OTase | - |
Campylobacter jejuni |
oligosaccharyltransferase | - |
Desulfovibrio desulfuricans |
oligosaccharyltransferase | - |
Campylobacter jejuni |
OTase | - |
Desulfovibrio desulfuricans |
OTase | - |
Campylobacter jejuni |
PglB | - |
Desulfovibrio desulfuricans |
PglB | - |
Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
evolution | in the delta proteobacteria Desulfovibrio sp., the PglB homologue is more closely related to eukaryotic and archaeal OTases than to its Campylobacter counterparts | Desulfovibrio desulfuricans |