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Literature summary for 2.5.1.10 extracted from

  • Takahashi, I.; Ogura, K.
    Farnesyl pyrophosphate synthetase from Bacillus subtilis (1981), J. Biochem., 89, 1581-1587.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,4-dithiothreitol 2 mM, 1.6fold stimulation Bacillus subtilis
2-mercaptoethanol 10 mM, 1.2fold stimulation Bacillus subtilis
NH4+ 3fold stimulation with geranyl diphosphate as substrate Bacillus subtilis
NH4+ 5fold stimulation with dimethylallyl diphosphate as substrate Bacillus subtilis
Triton X-100 0.5%, 3fold activation Bacillus subtilis
Tween 80 0.5%, 2.3fold activation Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
bacitracin
-
Bacillus subtilis
Ca2+ 5 mM, 80% inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
geranyl diphosphate
-
Bacillus subtilis
0.05
-
dimethylallyl diphosphate
-
Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates with geranyl diphosphate as substrate Bacillus subtilis
Mg2+ required for activity Bacillus subtilis
Mn2+ can partially replace Mg2+, less effective Bacillus subtilis
additional information not activated by Na+ or Li+ Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-100, hydroxylapatite, DEAE-Sephadex A-50 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
-
Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, at least 1 week, no loss of activity Bacillus subtilis
4°C, 5 days, 36% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
Bacillus subtilis (E,E)-farnesyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + isopentenyl diphosphate
-
Bacillus subtilis diphosphate + farnesyl diphosphate E)-farnesyl diphosphate ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Bacillus subtilis