Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,4-dithiothreitol | 2 mM, 1.6fold stimulation | Bacillus subtilis | |
2-mercaptoethanol | 10 mM, 1.2fold stimulation | Bacillus subtilis | |
NH4+ | 3fold stimulation with geranyl diphosphate as substrate | Bacillus subtilis | |
NH4+ | 5fold stimulation with dimethylallyl diphosphate as substrate | Bacillus subtilis | |
Triton X-100 | 0.5%, 3fold activation | Bacillus subtilis | |
Tween 80 | 0.5%, 2.3fold activation | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bacitracin | - |
Bacillus subtilis | |
Ca2+ | 5 mM, 80% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
geranyl diphosphate | - |
Bacillus subtilis | |
0.05 | - |
dimethylallyl diphosphate | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates with geranyl diphosphate as substrate | Bacillus subtilis | |
Mg2+ | required for activity | Bacillus subtilis | |
Mn2+ | can partially replace Mg2+, less effective | Bacillus subtilis | |
additional information | not activated by Na+ or Li+ | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-100, hydroxylapatite, DEAE-Sephadex A-50 | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, at least 1 week, no loss of activity | Bacillus subtilis |
4°C, 5 days, 36% loss of activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Bacillus subtilis | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | diphosphate + farnesyl diphosphate | E)-farnesyl diphosphate | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus subtilis |